Recombinant Mouse ADP-ribosylation factor-like protein 6 (Arl6)

Code CSB-YP002093MO
MSDS
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Source Yeast
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Code CSB-EP002093MO
MSDS
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Source E.coli
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Code CSB-EP002093MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002093MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002093MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arl6
Uniprot No.
Alternative Names
Arl6; Bbs3; ADP-ribosylation factor-like protein 6
Species
Mus musculus (Mouse)
Expression Region
2-186
Target Protein Sequence
GLLDRLSGL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQDIVPT IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKDGQAI IFVIDSSDKL RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDSVTS VKVSQLLCLE SIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQAVKT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. Together with BBS1, is necessary for correct trafficking of PKD1 to primary cilia. Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization.
Gene References into Functions
  1. IFT27 prevents aggregation of nucleotide-free ARL6 in solution PMID: 25443296
  2. Bbs3 knockout mouse model reveals common BBS-associated phenotypes and Bbs3 unique phenotypes PMID: 22139371
  3. These data demonstrate that the BBS3L transcript is required for proper retinal function and organization. PMID: 20333246
  4. ARL6 was expressed in the neural plate and probably in the somites PMID: 16172987
Subcellular Location
Cell projection, cilium membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, cilium basal body.
Protein Families
Small GTPase superfamily, Arf family
Tissue Specificity
Most abundant in brain and kidney. Expressed in heart and eye. Isoform 2 is expressed only in the retina.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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