Recombinant Mouse Amiloride-sensitive sodium channel subunit gamma (Scnn1g), partial

Code CSB-YP896238MO
MSDS
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Source Yeast
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Code CSB-EP896238MO
MSDS
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Source E.coli
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Code CSB-EP896238MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896238MO
MSDS
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Source Baculovirus
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Code CSB-MP896238MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Scnn1g
Uniprot No.
Alternative Names
Scnn1g; Amiloride-sensitive sodium channel subunit gamma; Epithelial Na(+) channel subunit gamma; Gamma-ENaC; Gamma-NaCH; Nonvoltage-gated sodium channel 1 subunit gamma; SCNEG
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Gene References into Functions
  1. Mouse cells conditionally overexpressing gamma ENAC had enhanced apical Na(+) entry increased the basolateral cell surface expression of Na,K-ATPase by inhibiting p38 kinase-mediated endocytosis of Na,K-ATPase. PMID: 24179170
  2. TMPRSS4 primarily activates ENaC by cleaving basic residues within the tract gammaK173-K186 distal to the furin cleavage site, thereby releasing a previously defined key inhibitory tract encompassing gammaR158-F168 from the gamma-subunit. PMID: 21993886
  3. estrogen regulates expression and function of epithelial sodium channel gamma in osteoblasts PMID: 21624334
  4. Il-6 activates ENaC-gamma channels in cortical collecting duct cells. PMID: 20504903
  5. residues within Gln573-Arg583 of gammaENaC are necessary for normal channel gating PMID: 12897071
  6. Scnn1g is a PPARgamma target gene in the renal collecting duct. PMID: 16007095
  7. Aldosterone-induced transcriptional up-regulation of the channel's beta- and gamma-subunit (beta ENaC and gamma ENaC) and of the serum- and glucocorticoid-inducible kinase 1 (SGK1) was similar in colon tissue from Liddle and wild-type animals PMID: 18006588
  8. SGK1 is essential for optimal processing of ENaC but is not required for activation of the channel by aldosterone PMID: 18385268
  9. P2Y2 receptor activation by nucleotides thus provides physiologically important regulation of ENaC and electrolyte handling in mammalian kidney PMID: 18981175
  10. Plasmin activates epithelial Na+ channels by cleaving the gamma subunit PMID: 18981180

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Subcellular Location
Apical cell membrane; Multi-pass membrane protein.
Protein Families
Amiloride-sensitive sodium channel (TC 1.A.6) family, SCNN1G subfamily
Tissue Specificity
Lung and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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