Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ankrd2

Uniprot No.

Q9WV06

Alternative Names

Ankrd2; ArppAnkyrin repeat domain-containing protein 2; Skeletal muscle ankyrin repeat protein; mArpp

Species

Mus musculus (Mouse)

Expression Region

1-358

Target Protein Sequence

MTKAPSGRGG CLGLKRPHAL WLAETVMEGT MEGPEAVQRA TELIEQRLAQ EEETEKLRRS APGKLSMDML VLEEEKRLGV QSPALQKVKG QERVRKTSLD LRREIIDVGG IQNLIELRKK RKQKKRDALA AAQEPPPEPE EITGPVNEET FLKAAVEGKM KVIDKYLADG GSADTCDEFR RTALHRASLE GHMEILEKLL ENGATVDFQD RLDCTAMHWA CRGGHLEVVR LLQSRGADTN VRDKLLSTPL HVAVRTGHVE IVEHFLSLGL DINAKDREGD SALHDAVRLN RYKIIKLLLL HGADMMAKNL AGKTPTDLVQ LWQADTRHAL EHPEPESEQN GLERPGSGRE TPQPIPAQ

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions as a negative regulator of myocyte differentiation. May interact with both sarcoplasmic structural proteins and nuclear proteins to regulate gene expression during muscle development and in response to muscle stress.

Gene References into Functions

We describe a novel mechanism of signal transduction enriched in medium spiny neurons of striatum that likely mediates effects of the neurotransmitter dopamine acting on these cells. We find that the protein ARPP-16, which is highly expressed in striatal medium spiny neurons, acts as a selective inhibitor of certain forms of the serine/threonine protein phosphatase, PP2A, when phosphorylated by the kinase, MAST3. PMID: 28167675
CARP, Ankrd2, and DARP are not essential for normal cardiac development and function at basal conditions and in response to mechanical pressure overload. PMID: 24736439
Ankrd2 as a potent repressor of inflammatory responses through direct interaction with the NF-kappaB repressor subunit p50. PMID: 24434510
a novel pathway in which SREBP-1/ANKRD2/ID3 activation inhibits myoblast differentiation, and we propose that this pathway acts as a critical determinant of the skeletal muscle developmental program. PMID: 23824195
assignment to mouse chromosome 19C3 PMID: 15222314
We suggest that Ankrd2, which is also a titin binding protein, is a stretch-response gene associated with slow muscle function and that it is part of a separate mechanotransduction system to the one that regulates muscle mass. PMID: 15677738
Ankrd2 gene expression was analyzed at three different stages in differentiating MyoD(-)C2C12 myoblasts PMID: 15890200
Ankrd2 plays important structural and gene regulatory roles in skeletal muscle. PMID: 17392382
The Ankrd2 accumulated in the nuclei of myofibers located adjacent to severely damaged myofibers after muscle injury. Ankrd2 may translocate from the I-band to the nucleus in response to muscle damage and in the regulation of gene expression. PMID: 17926058

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Subcellular Location

Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Note=In the sarcoplasm of differentiated striated muscle cells, where it is cytosolic and enriched in the I band. In nucleus and PML bodies of proliferating and undifferentiated myoblasts. Associates with the euchromatin in the nucleus of myocytes upon muscle stress.

Tissue Specificity

Expressed by myoblasts (at protein level). Expressed in skeletal and cardiac muscles.

Database Links

KEGG: mmu:56642

STRING: 10090.ENSMUSP00000026172

UniGene: Mm.143737

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Recombinant Mouse Ankyrin repeat domain-containing protein 2 (Ankrd2)

Product Details

Customer Reviews and Q&A

Target Background

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