Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Bag3

Uniprot No.

Q9JLV1

Research Area

Others

Alternative Names

Bag3; Bis; MNCb-2243BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-577aa

Target Protein Sequence

SAATQSPMMQMASGNGASDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPPEGPKDTASSANGPSRDGSRLLPIREGHPIYPQLRPGYIPIPVLHEGSENRQPHLFHAYSQPGVQRFRTEAAAATPQRSQSPLRGGMTEAAQTDKQCGQMPATATTAAAQPPTAHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYIPIPVIHEQNITRPAAQPSFHQAQKTHYPAQQGEYQPQQPVYHKIQGDDWEPRPLRAASPFRSPVRGASSREGSPARSGTPVHCPSPIRVHTVVDRPQPMTHREPPPVTQPENKPESKPGPAGPDLPPGHIPIQVIRREADSKPVSQKSPPPAEKVEVKVSSAPIPCPSPSPAPSAVPSPPKNVAAEQKAAPSPAPAEPAAPKSGEAETPPKHPGVLKVEAILEKVQGLEQAVDSFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEAEQPLQEIMGAVVADKDKKGPENKDPQTESQQLEAKAATPPNPSNPADSAGNLVAP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

77.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.

Gene References into Functions

expression of the co-chaperone BAG3 and other chaperone-assisted selective autophagy factors was analyzed in the cellular, zebrafish and ky/ky mouse models. PMID: 29914939
It is a cochaperone that mediates autophagy. PMID: 29304346
Thus, BAG3 is critical for the protein turnover of small HSPs via activation of autophagy in the heart. PMID: 29409895
BAG3 plays a relevant role in regulating SNCA clearance via macroautophagy, and the heat shock protein 70-BAG3-sequestosome 1 complex may be involved in this process. PMID: 28941726
BAG3 directly stabilizes hexokinase 2 mRNA and promotes aerobic glycolysis in pancreatic cancer cells. PMID: 29114069
BAG3 expression is required for neuronal differentiation and migration. PMID: 28144784
interaction between BAG3 and HSP70 is essential for BAG3 to stabilize small heat shock proteins and maintain cardiomyocyte protein homeostasis PMID: 28737513
The spatial regulation of mTORC1 exerted by BAG3 apparently provides the basis for a simultaneous induction of autophagy and protein synthesis to maintain the proteome under mechanical strain. PMID: 27756573
Genetic variation in BAG3 plays an important role in the prevention of ischemic tissue necrosis. PMID: 28442482
The aim of this study was to investigate the possible hemodynamic effects of BAG3 performing both in vitro and in vivo experiments. PMID: 27763645
Our findings that BAG3 is localized at the sarcolemma and t-tubules while modulating myocyte contraction and action potential duration through specific interaction with the beta1-adrenergic receptor and L-type Ca(2+) channel provide novel insight into the role of BAG3 in cardiomyopathies and increased arrhythmia risks in heart failure. PMID: 26796036
molecular association of MyHC and BIS is necessary for MyHC stabilization in skeletal muscle. PMID: 27034027
BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages. PMID: 26522614
Bis is upregulated in astrocytes after hypoxia-ischemia; hypoxia-ischemia induces progressive cell death in the hippocampi of bis-positive mice, while hippocampal neurons are less vulnerable to hypoxia-ischemia in mice that lack Bis. PMID: 22907804
Deletion of the bis gene results in a marked increase in the production of corticosterone that is associated with thymic atrophy. PMID: 21540452
BAG3 and Hsc70 interact with actin capping protein CapZ to maintain myofibrillar integrity under mechanical stress. PMID: 20884878
Results indicate that Bis functions to mediate cellular regulation of the stem cell niche on the vascular compartment. PMID: 20024912
BAG3 alters the interaction between HSP70 and IKKgamma, increasing availability of IKKgamma and protecting it from proteasome-dependent degradation; this, in turn, results in increased NF-kappaB activity and survival PMID: 20368414
BAG3 is not required for muscle development, this co-chaperone appears to be critically important for maintenance of mature skeletal muscle. PMID: 16936253
The absence of Bis has considerable influences on postnatal growth and survival, possibly due to a nutritional impairment. PMID: 18840758

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Subcellular Location

Nucleus. Cytoplasm.

Database Links

KEGG: mmu:29810

STRING: 10090.ENSMUSP00000033136

UniGene: Mm.84073

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Code	CSB-EP864372MO
MSDS	MSDS
Size	US$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP864372MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Bag3. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP864372MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Bag3.
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Recombinant Mouse BAG family molecular chaperone regulator 3 (Bag3)

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