Recombinant Mouse BarH-like 2 homeobox protein (Barhl2)

Code CSB-YP848716MO
MSDS
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Source Yeast
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Code CSB-EP848716MO
MSDS
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Source E.coli
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Code CSB-EP848716MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848716MO
MSDS
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Source Baculovirus
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Code CSB-MP848716MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Barhl2
Uniprot No.
Alternative Names
Barhl2; Barh1; Mbh1BarH-like 2 homeobox protein; Bar-class homeodomain protein MBH1; Homeobox protein B-H1
Species
Mus musculus (Mouse)
Expression Region
1-384
Target Protein Sequence
MTAMEGASGS SFGIDTILSG AGSGSPGMMN GDFRSLGEAR TTDFRSQATP SPCSEIDTVG TAPSSPISVT LEPPEPHLVT DGPQHHHHLH HSQQPPPPSA VPAQSLQPSP QQQPPPQPQS AAQQLGSAAA APRTSTSSFL IKDILGDSKP LAACAPYSTS VSSPHHTPKQ ESNAAHESFR PKLEQEDGKT KLDKREDPQS DIKCHGTKEE GDREITSSRE SPPVRAKKPR KARTAFSDHQ LNQLERSFER QKYLSVQDRM DLAAALNLTD TQVKTWYQNR RTKWKRQTAV GLELLAEAGN YSALQRMFPS PYFYHPSLLG SMDSTTAAAA AAAMYSSMYR TPPAPHPQLQ RPLVPRVLIH GLGPGGQPAL NPLSNPIPGT PHPR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Potential regulator of neural basic helix-loop-helix genes. It may down-regulate expression of ASCL1 and, within the thalamus, up-regulate NGN2, thereby regulating distinct patterns of neuronal differentiation.
Gene References into Functions
  1. by acting upstream of Shh signaling pathway, Barhl2 plays a crucial role in patterning the progenitor domains and establishing the positional identities of progenitor cells in the diencephalon. PMID: 27349434
  2. The expression of Barhl2 in the thalamus and pretectum is related to, and regulated by, the expression of Pax6. Pax6 is known to be required for normal diencephalic development. The findings suggest that some of its actions might be mediated by its maintenance of a repressive influence over Barhl2 expression in the thalamus and pretectum. PMID: 27887593
  3. results elucidate BARHL2 as a critical postmitotic regulator of dI1 subtype diversification, as well as its intermediate position in the dI1 genetic hierarchy PMID: 22307612
  4. in the developing diencephalon, Caspase3 acts downstream of Barhl2 in limiting neuroepithelial cell proliferation by inhibiting beta-catenin activation. PMID: 21262809
  5. Data suggest that Barhl2 may function to specify the identity of glycinergic amacrine cells from competent progenitors during retinogenesis. PMID: 14998930
  6. Math1 directly regulates the Mbh genes in CGCs. Furthermore, a dominant-negative form of the Mbh proteins disrupted proper PMID: 18723012
  7. BARHL2 plays roles in retinal development, including regulating neuronal subtype specification, differentiation, and survival. PMID: 19339595

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Subcellular Location
Nucleus.
Protein Families
BAR homeobox family
Database Links
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