Recombinant Mouse DENN domain-containing protein 1B (Dennd1b)

Code CSB-YP662324MO
MSDS
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Source Yeast
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Code CSB-EP662324MO
MSDS
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Source E.coli
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Code CSB-EP662324MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP662324MO
MSDS
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Source Baculovirus
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Code CSB-MP662324MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dennd1b
Uniprot No.
Alternative Names
Dennd1bDENN domain-containing protein 1B; Connecdenn 2
Species
Mus musculus (Mouse)
Expression Region
1-425
Target Protein Sequence
MDRRTRENPE RTFDLVLKVK CHASENEDPE VLWKFPEDFG DQEVLQSVPK FCFPFDVERV SQNQVGQHFT FVLTDMESKQ RFGFCRLTSG GRICLCILSY LPWFEVYYKL LNTLADYLAK ELEEDLNETL KSLYNHPVPK ANTPVNLSVN QELFIASEQV LKDDPSLIPH SCFITPDITG LPTIPESRNL TEYFVAVDVN NMLRLYASML HERRIIITSS KLSTLTACLH GSAALLYPMY WQHIYIPVLP PHLLDYCCAP MPYLIGIHSS LIERVKNKSL EDVVVLNVDT NTLESPFNDL SSLPSDVVSA LKNKLKKQST ATGDGVARAF LRAQAALFGS YRDALRYKPG EPITFCEESF VKHRSSVMKQ FLETAVNLQL FKQFIDGRLA KLNAGRGFSD IFEEEITSGG FCGGKDSLES NYLDI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells.
Gene References into Functions
  1. Dennd1b(-/-) mice were generated and exhibit hyper-allergic responses following antigen challenge. Dennd1b(-/-) TH2, but not other TH cells, exhibit delayed receptor-induced T cell receptor (TCR) downmodulation, enhanced TCR signaling, and increased production of effector cytokines. PMID: 26774822
Subcellular Location
Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.
Tissue Specificity
Expressed in a subset of dendritic cells (DCs).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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