Recombinant Mouse DNA repair protein XRCC2 (Xrcc2)

Code CSB-YP866622MO
MSDS
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Source Yeast
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Code CSB-EP866622MO
MSDS
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Source E.coli
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Code CSB-EP866622MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866622MO
MSDS
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Source Baculovirus
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Code CSB-MP866622MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Xrcc2
Uniprot No.
Alternative Names
Xrcc2; DNA repair protein XRCC2; X-ray repair cross-complementing protein 2
Species
Mus musculus (Mouse)
Expression Region
1-278
Target Protein Sequence
MCSDFRRAES GTELLARLEG RSSLKELEPN LFADEDSPVH GDIFEFHGPE GTGKTEMLYH LTARCILPKS EGGLQIEVLF IDTDYHFDML RLVTVLEHRL SQSSEEAMKL CLARLFLAYC SSSMQLLLTL HSLEALLCSR PSLCLLIVDS LSSFYWIDRV SGGESVALQE STLQKCSQLL ERLVTEYRLL LFATTQSLMQ KGSDSADGPS SSKHPCDGDM GYRAYLCKAW QRVVKHRVIF SRDDEAKSSR FSLVSRHLKS NSLKKHSFMV RESGVEFC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Gene References into Functions
  1. involvement of ZNF281 in the cellular response to genotoxic stress through the control exercised on the expression of genes that act in different repair mechanisms PMID: 26300006
  2. Data show that XRCC2 and other homologous recombination proteins, including the key recombinase RAD51, co-localize with the centrosome, potentially to coordinate the deployment of a DNA damage response at vulnerable phases of the cell cycle. PMID: 21276791
  3. These findings indicate that Xrcc2 haploinsufficiency reduces spontaneous tumor incidence on an Apc(min/+) background but increases the tumorigenic response to radiation. PMID: 20671066
  4. An important function of XRCC2 is to enhance the activity of RAD51, so that the loss of XRCC2 results in a severe delay in the early response of RAD51 to DNA damage. PMID: 20189471
  5. Order-of-magnitude higher levels of chromosomal alterations are sustained in primary or immortal Xrcc2(-/-) embryonic fibroblasts. PMID: 14678973
  6. the normal developmental programme is perturbed in Xrcc2-/- embryonic tissues, particularly during neurogenesis and somitogenesis PMID: 17116431
  7. ATP hydrolysis by Rad51 is more important for some homologous recombination functions than it is for other aspects of DNA repair PMID: 17719855
  8. Xrcc2 is important to preserve or restore replication forks during rapid clonal expansion of developing lymphocytes. PMID: 18212067
  9. DNA replication licensing factor MCM2 and DNA mismatch repair (MMR) protein MSH2 were confirmed to be present in co-precipitations with MmXRCC2. PMID: 19658102

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Subcellular Location
Nucleus.
Protein Families
RecA family, RAD51 subfamily
Tissue Specificity
Expressed at low level in somatic tissues and at high level in testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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