Recombinant Mouse Dapper homolog 2 (Dact2)

Code CSB-YP752630MO
MSDS
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Source Yeast
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Code CSB-EP752630MO
MSDS
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Source E.coli
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Code CSB-EP752630MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP752630MO
MSDS
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Source Baculovirus
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Code CSB-MP752630MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dact2
Uniprot No.
Alternative Names
Dact2; Dpr2; Frd2; Dapper homolog 2; mDpr2; Dapper antagonist of catenin 2
Species
Mus musculus (Mouse)
Expression Region
1-757
Target Protein Sequence
MWAPSGQGPA GWDRRRVGAR LRAALAGLQE LQGLRATQQA RVRGALGLHP APGPRGQELR LEAALTALRE QLSRLRRQDA GLKTHLDQLD QQISELQLDV SRSSCEALDS DSRPSSGFYE LSDAGSCSLS TSCASVCSDR LSPSLGSWLP VFQPSKSRSG IGDWRPRSAD ETTVPAWSPQ LTEDSRLLHG AEGTGRLTGM FRPRPVSTGD LERVLPADVG LQRAGTDAAH LLGQGIEIPA HALDPTYQRD LVARGGQEVY PYPSPLHAVA LQSPLFALPK EAPCFDICSP PQEPPLVPVD ENRTQPEPIR ELGSAEAYIH RLLHLRGQEL PLRDVGQEQG GDTAAFPPKP CGQRSESTCQ LEKQACGADR GGLKLGRGAA KDSLKQHGPV SLVGAEPLSS PLKEETIPWN PCVHGDNTVG SSPCSQAQQP LNDCGQGPVL SPSRVLGTES PPLAPEPFAY TSCTTGETSP VKLRMGFSQN KAVKVRRRVS EKVPRLGKQL PPQPERQRVT ERDPSRPHQG GLSRRPTLAR EPPGRSCSES TLYPVPFLVP VVVAQRESYP TSPQAFFPME AALLSSAARR KQRRWQSTME ISAKAGSVSQ PGPSMGLPRS PAKRGSGPRA QSRPTLARQD ACARCESDPS EHSADCTSLY HSTIAETSED EEASDHTANR FGDESSSNDS EGCFRGSRRR LAIGSAEAGQ GGWAWPRVPP QQPSRAPGNT RPPLPPVPKL CRIKASKALK KKIRRFQPAA LKVMTMV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling.
Gene References into Functions
  1. we have identified a novel role for Dact2 as an inhibitor of the canonical Wnt pathway in embryonic tooth development through its regulation of cell proliferation and differentiation. PMID: 23349981
  2. conserved role for Dact2 protein in kinase-regulated biochemistry involving Vangl and Dvl. PMID: 21718540
  3. Dact2 plays a role in regulating morphogenesis by renal collecting duct cells, probably by protecting cells from overly strong TGF-beta pathway activation. PMID: 20685821
  4. Dact2 may play important signal-modulating roles in the adjacent epithelial cells including the enamel knot signaling centers and pre-ameloblasts. PMID: 20170752
  5. Dact2 expression is most prominent during organogenesis of the thymus, kidneys, and salivary glands, with much lower levels in the somites and in the developing CNS. PMID: 16881060
  6. Dpr2 plays an inhibitory role in the re-epithelialization of adult skin wounds by attenuating TGFbeta signaling. PMID: 18716284

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Protein Families
Dapper family
Tissue Specificity
Expressed in kidney (inner medullary collecting duct). Expressed in epidermal keratinocytes and hair follicles.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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