Recombinant Mouse E3 ubiquitin-protein ligase rififylin (Rffl)

Code CSB-YP751168MO
MSDS
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Source Yeast
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Code CSB-EP751168MO
MSDS
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Source E.coli
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Code CSB-EP751168MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP751168MO
MSDS
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Source Baculovirus
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Code CSB-MP751168MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rffl
Uniprot No.
Alternative Names
Rffl; E3 ubiquitin-protein ligase rififylin; EC 2.3.2.27; RING finger and FYVE-like domain-containing protein 1; Fring; RING-type E3 ubiquitin transferase rififylin
Species
Mus musculus (Mouse)
Expression Region
1-377
Target Protein Sequence
MSQPSLWKDS HYFIMWASCC NWFCLDGQPE EAPPPQGART QAYSNPGYSS FPSPTGSEPS CKACGVHFAS TTRKQTCLDC KKNFCMTCSS QEGNGPRLCL LCLRFRATAF QREELMKMKV KDLRDYLSLH DISTEMCREK EELVFLVLGQ QPVISEADRT RVPHLPQAFP EQQAFLTQPQ TSTVPPTSPG LPSSPAQVTS VPLAQDQETQ QAVGHVSQDH EEPIFPESTA RVPTEDETQS VDSEDSFVPG RRASLSDLTH LEDIEGLTVR QLKEILARNF VNYKGCCEKW ELMERVTRLY KDQKGLQHLV SGNEDQNGGA VPSGLEENLC KICMDSPIDC VLLECGHMVT CTKCGKRMNE CPICRQYVIR AVHVFRS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
Gene References into Functions
  1. EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression. PMID: 24114843
  2. Data show that the the amino-terminal domain of rififylin is critical for its recruitment to recycling endocytic membranes and for the inhibition of recycling. PMID: 15229288
  3. CARP2 [Rififylin] deficiency does not alter induction of NF-kappaB by TNFalpha. PMID: 19138581
Subcellular Location
Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate-binding and control subcellular localization.
Tissue Specificity
Ubiquitous. Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney, testis, thymus, whole embryo and embryonic stem cells.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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