Recombinant Mouse EP300-interacting inhibitor of differentiation 1 (Eid1)

Code CSB-YP887598MO
MSDS
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Source Yeast
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Code CSB-EP887598MO
MSDS
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Source E.coli
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Code CSB-EP887598MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887598MO
MSDS
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Source Baculovirus
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Code CSB-MP887598MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Eid1
Uniprot No.
Alternative Names
Eid1; Cri1EP300-interacting inhibitor of differentiation 1; CREBBP/EP300 inhibitory protein 1; E1A-like inhibitor of differentiation 1; EID-1
Species
Mus musculus (Mouse)
Expression Region
1-169
Target Protein Sequence
MAEMAELCEL YEESNELQMD VLPGEGYMEV GRGARGPAPE EGPMEEEAGP AAARAQRGLF PEAGADLEGD EFDDWEDDYE FPEEERWSGA MHRVSAALEE ANKVFLRTAR AGDALDGGFQ ARCEKSPFDQ LAFIEELFSL MVVNRLTEEL GCDEIIDREL MLTREEETT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
Gene References into Functions
  1. palmitoylation modified E1A-like inhibitor of differentiation one (EID1) and this modification regulated EID1 degradation and CREB-binding protein (CBP)/p300 histone acetyltransferase activity at the switch between self-renewal and differentiation of neural stem cells. PMID: 26497028
  2. Pcid2 is present in the CBP/p300-EID1 complex to control the switch balance of mouse and human embryonic stem cells through modulation of EID1 degradation. PMID: 24167073
  3. This study supports Cri1 as the major locus controlling susceptibility following murine infection with C. rodentium, and suggests a cause of mortality common to the susceptible C3, C3Ou and FVB strains of mice. PMID: 21804358
  4. Cri1 does not control C. rodentium colonic hyperplasia PMID: 21326319
  5. EID-1 is expressed in the mouse testis, and its expression decreases during testis development. The results of the present study suggest that EID-1 can act as a repressor, regulating the function of SF-1. PMID: 18182853

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Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed in all adult tissues examined and during embryogenesis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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