Product Details

Purity

>85% (SDS-PAGE)

Target Names

Eppk1

Uniprot No.

Q8R0W0

Alternative Names

Eppk1; EPPKEpiplakin

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Cytoskeletal linker protein that connects to intermediate filaments and controls their reorganization in response to stress. In response to mechanical stress like wound healing, is associated with the machinery for cellular motility by slowing down keratinocyte migration and proliferation and accelerating keratin bundling in proliferating keratinocytes thus contributing to tissue architecture. However in wound healing in corneal epithelium also positively regulates cell differentiation and proliferation and negatively regulates migration thereby controlling corneal epithelium morphogenesis and integrity. In response to cellular stress, plays a role in keratin filament reorganization, probably by protecting keratin filaments against disruption. During liver and pancreas injuries, plays a protective role by chaperoning disease-induced intermediate filament reorganization.

Gene References into Functions

Epiplakin is a protective protein during acute pancreatitis, and that its loss causes impaired disease-associated keratin reorganization. PMID: 25232867
In several liver stress models epiplakin and K8 genes displayed identical expression patterns and transgenic K8 overexpression resulted in elevated hepatic epiplakin levels. PMID: 25617501
Lacking epiplakin affects cell differentiation of the corneal epithelium, as well as its proliferation activity and its structural integrity. PMID: 23599337
Eppk1 serves as a useful marker for detecting the hepatic progenitor population in the developing and adult liver PMID: 21216305
These results suggest that Eppk1 is expressed both in embryonic and adult retina and may be involved in retinal development. PMID: 20151282
characterization of the epiplakin gene locus and the isolation of full-length epiplakin cDNA using BAC vectors; expression is restricted to epithelial tissues and its gene maps to mouse chromosome 15 PMID: 12791695
EPPK might be linked functionally with keratin 6. PMID: 16382146
Serves as a useful marker for detecting pancreatic progenitor cells in developing and regenerating pancreas. PMID: 18498355

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Subcellular Location

Cytoplasm, cytoskeleton. Apicolateral cell membrane. Basolateral cell membrane. Cell junction. Cell junction, hemidesmosome. Cell junction, tight junction. Cell projection.

Protein Families

Plakin or cytolinker family

Tissue Specificity

High levels in skin, small intestine and salivary gland. Lower levels in lung, uterus and liver. Not detected in brain, kidney, muscle, heart or spleen. In skin, expressed in all epidermal layers but not in the dermis. In intestine, expressed exclusively

Database Links

KEGG: mmu:223650

UniGene: Mm.259929

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Recombinant Mouse Epiplakin (Eppk1), partial

Product Details

Customer Reviews and Q&A

Target Background

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