Recombinant Mouse F-box only protein 33 (Fbxo33)

Code CSB-YP855339MO
MSDS
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Source Yeast
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Code CSB-EP855339MO
MSDS
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Source E.coli
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Code CSB-EP855339MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855339MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP855339MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fbxo33
Uniprot No.
Alternative Names
Fbxo33F-box only protein 33; AIG30-12-1
Species
Mus musculus (Mouse)
Expression Region
1-562
Target Protein Sequence
MLLFLSVPQP RPPGARTRAG AARLVRWRRR QRLRLLQLRR LRGLLRGLRR RPGTGGRRPS RMALCGQAAG AASLPSELIV HIFSFLPAPD RLRASASCSH WRECLFYPAL WPQLRICLRV SPAEQPRLEF LMRKCGWFVR ELRVEFAAEN YLSGGGGPGD GGSGGGTDTG TGGEDGEALQ LSSRWLEVLR IYLELVLCVL LSIRNNRNLQ KFSLFGDISV VHQQGSLSST YLSRVDPDGK KIKQIQQLFE EILSNSRQLK WLSCGFMLEI VTPTSLSSLS NPIANTMEHL SLLDNNIPGN STLITAVELE RFVNLRSLAL DFCDFTAEMA RVLTDSNHVP LQRLSLLVHN ASVMLKSLDN MPNDEHWKAL SRKSSSLRVY LMVFDIKSED MLKILKPSIP LERVHFDSYV TCVSGAIVDL ISRQYDKFLT HFILMNDMID TSGFPDLSDN RNEDPLVLLA WRCTKLTLLA IHGYTVWAHN LIAIARLRGS DLKVLQVTEE SIDFDQGELA DQDVDPVQNL IEQVSLGLGQ SWHAVLDIES LSVFTEPNRH FYREMQSFSE DI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.
Gene References into Functions
  1. mRNA expression in the hippocampus 1 h following generalized convulsive seizure, in a pattern which is typical for other activity-dependant genes expressed in this structure. PMID: 15380003
  2. FBX33 as a component of an SCF E3-ubiquitin ligase that targets the multifunctional regulator Y-box binding protein 1 (YB-1)/dbpB/p50 for polyubiquitination and destruction by the proteasome. PMID: 16797541
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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