Recombinant Mouse F-box only protein 9 (Fbxo9)

Code CSB-YP804362MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP804362MO
MSDS
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Source E.coli
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Code CSB-EP804362MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804362MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP804362MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fbxo9
Uniprot No.
Alternative Names
Fbxo9; MNCb-2471F-box only protein 9
Species
Mus musculus (Mouse)
Expression Region
1-437
Target Protein Sequence
MSAEAEEDCH SDADRVGDEG NESPAERDLQ AQLQMFRAQW MFELTPGVGS SHGETRPCRA GRSSMLKAAA DTKGRQELAK EEKARELFLQ AVEEEQNGAL YEAIKFYRRA MQLVPDIEFK ITYTRSPDGD GVGSGYIEEN EDASKMADLL SYFQQQLTLQ ESVLKLCQPE LETSQTHISV LPMEVLMYIF RWVVSSDLDL RSLEQLSLVC RGFYICARDP EIWRLACLKV WGRSCMKLVP YASWREMFLE RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYM RFFPDGHVMM LTTPEEPPSI VPRLRTRNTR TDAILLGHYR LSQDADNQTK VFAVITKKKE EKPLDHKYRY FRRVPVQEAD HSFHVGLQLC SSGHQRFNKL IWIHHSCHIT YKATGETAVS AFEIDKMYTP LLFARVRSYT AFSERPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.
Gene References into Functions
  1. FBXO9 directly interacted with PPAR gamma through the activation function-1 domain and ligand-binding domain. FBXO9 decreased the protein stability of PPAR gamma through induction of ubiquitination. PMID: 27197753
  2. ICK and FBX9 are divergently transcribed from a bidirectional promoter that is GC-rich and contains a CpG island. PMID: 20459822
Subcellular Location
Cytoplasm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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