Recombinant Mouse FH1/FH2 domain-containing protein 3 (Fhod3), partial

Code CSB-YP758839MO
MSDS
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Source Yeast
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Code CSB-EP758839MO
MSDS
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Source E.coli
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Code CSB-EP758839MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP758839MO
MSDS
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Source Baculovirus
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Code CSB-MP758839MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fhod3
Uniprot No.
Alternative Names
Fhod3; Fhos2; Kiaa1695FH1/FH2 domain-containing protein 3; Formin homolog overexpressed in spleen 2; mFHOS2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in actin filament polymerization in cardiomyocytes. Actin-organizing protein that may cause stress fiber formation together with cell elongation.
Gene References into Functions
  1. Perinatal KO of Fhod3 in the heart caused juvenile lethality at around day 10 after birth with enlarged hearts composed of severely impaired myofibrils, indicating that Fhod3 is crucial for postnatal heart development. PMID: 29158260
  2. these findings suggest the possibility that Fhod3 contributes to the pathogenesis of cMyBP-C-related cardiomyopathy and that Fhod3 is critically involved in cMyBP-C-mediated regulation of cardiac function via direct interaction. PMID: 29686099
  3. Up regulation of FHOD3 play a crucial role in the pathogenesis of cardiac hypertrophy in mouse model. PMID: 28438902
  4. during embryonic cardiogenesis, Fhod3 functions as the essential reorganizer of actin filaments at the central region of maturating sarcomeres via the actin-binding activity of the FH2 domain PMID: 26848968
  5. Analysis of casein kinase 2 alpha knockout mice showed that phosphorylation by casein kinase 2 is also required for proper targeting of muscle Fhod3 to the myofibrils in embryonic cardiomyocytes in situ. PMID: 23052206
  6. embryonic heart of mice specifically expresses the Fhod3 mRNA isoform harboring the three alternative exons PMID: 22509354
  7. actin dynamics regulated by Fhod3 are critical for sarcomere organization in striated muscle cells. PMID: 19706596

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line.
Protein Families
Formin homology family
Tissue Specificity
Expressed in the heart, including left ventricle, kidney, brain and skeletal muscle, including soleus and tibialis anterior (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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