Recombinant Mouse Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), partial

Code CSB-YP623957MO
MSDS
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Source Yeast
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Code CSB-EP623957MO
MSDS
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Source E.coli
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Code CSB-EP623957MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP623957MO
MSDS
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Source Baculovirus
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Code CSB-MP623957MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gpat2
Uniprot No.
Alternative Names
Gpat2; Glycerol-3-phosphate acyltransferase 2; mitochondrial; GPAT-2; EC 2.3.1.15; xGPAT1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transfers an acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate producing a lysophosphatidic acid (LPA), an essential step for the triacylglycerol (TAG) and glycerophospholipids. In vitro also transfers an acyl-group from acyl-ACP to the LPA producing a phosphatidic acid (PA). Prefers arachidonoyl-CoA as the acyl donor. Required for primary processing step during piRNA biosynthesis. Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity.
Gene References into Functions
  1. Data suggest that Gpat2 is necessary for normal spermatogenesis; absence of Gpat2 up-regulates expression of apoptosis regulatory proteins, triggers apoptosis, and thereby decreases the number of dividing germ cells; Gpat2 knockdown (using RNA silencing) prevents germ cells from proceeding through pachytene stage. PMID: 28729426
  2. Thus, Gpat2 is expressed at a specific stage of spermatogenesis, consistent with piRNA synthesis and meiosis I prophase PMID: 26268560
  3. GPAT2 is essential for the biogenesis of piRNA. PMID: 23611983
  4. GPAT2 expression is linked to arachidonoyl-CoA incorporation into TAG in spermatogenic germ cells PMID: 22905194
  5. These results suggest that the GPAT2 protein has two transmembrane segments and that the N-terminal and C-terminal regions of this protein face the cytoplasm. PMID: 22285183
  6. A novel murine glycerol-3-phosphate acyltransferase-like protein 1 (named xGPAT1) has been cloned. PMID: 17013544

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Subcellular Location
Mitochondrion outer membrane; Multi-pass membrane protein.
Protein Families
GPAT/DAPAT family
Tissue Specificity
Highly expressed in the testis. Expressed at lower levels in the heart, liver, kidney, spleen and adipose cells. Only detected in primary spermatocytes.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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