Recombinant Mouse Hematopoietic SH2 domain-containing protein (Hsh2d)

Code CSB-YP744289MO
MSDS
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Source Yeast
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Code CSB-EP744289MO
MSDS
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Source E.coli
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Code CSB-EP744289MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744289MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP744289MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hsh2d
Uniprot No.
Alternative Names
Hsh2d; Alx; Hematopoietic SH2 domain-containing protein; Hematopoietic SH2 protein; Adaptor in lymphocytes of unknown function X
Species
Mus musculus (Mouse)
Expression Region
1-334
Target Protein Sequence
MAEARRLPPP LPPRLDWFVH TQADLLAQSG IPEWFHGTIS REAAENMLES QPLGTFLIRV SHSHVGYTLS YKAQTCCRHF MVKLSEDGTC AFAGDHMTHA SLHALVTFHQ QKPIRPFGEL LTQACGQEDP ANVDYEDLFL YSNALVQDAE SQILRTEVQR SSCPPEEASE RKPSTTTKGE FASASCSPKA LFEDSGQKLW KNLRSLPQTS QRVKQRLTSH LLAMNLLGDA RQVAQQHHSP VTRAFSWDST SHSEDSCAAT TSLQNPAEPQ ALRGREATFR DSRPASWRKA FSGVKAWRGK VVRALSAQEP VDFPEAQGWL PEEYLPPPPF APGY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter protein involved in tyrosine kinase and CD28 signaling. May be a modulator of the apoptotic response through its ability to affect mitochondrial stability.
Gene References into Functions
  1. These data demonstrate that changes in HSH2 expression have profound effects on the humoral immune response. PMID: 21873522
  2. ALX is an adaptor involved in T cell activation and interleukin-2 (IL-2) promoter activation PMID: 12960172
  3. endogenous HSH2 expression correlates directly with survival of WEHI-231 cells, which supports the hypothesis that HSH2 modulates the apoptotic response through its ability to directly or indirectly promote mitochondrial stability PMID: 15569688
  4. HSH2 expression is coordinately up-regulated with known antiapoptotic molecules and directly correlates with B cell survival PMID: 16547253
  5. These results suggest that ALX, in association with LAX, negatively regulates T-cell activation through inhibition of p38. PMID: 16880512
  6. Although adaptor in lymphocytes of unknown function X (ALX) is coexpressed with related adaptor Rlk/Itk-binding protein (RIBP) on both T- and B-lymphocytes throughout cell development, no redundancy or synergy is found. PMID: 17641043

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Subcellular Location
Cytoplasm. Mitochondrion.
Tissue Specificity
Predominantly expressed in spleen and thymus. Appears not to be expressed in heart, brain, liver, kidney, embryo, lung and ovary.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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