Recombinant Mouse Histone-lysine N-methyltransferase EZH1(Ezh1)

Code CSB-YP007912MO
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Source Yeast
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Code CSB-EP007912MO
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Source E.coli
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Code CSB-EP007912MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007912MO
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Source Baculovirus
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Code CSB-MP007912MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ezh1
Uniprot No. P70351
Alternative Names Ezh1; Enx2Histone-lysine N-methyltransferase EZH1; EC 2.1.1.356; ENX-2; Enhancer of zeste homolog 1
Species Mus musculus (Mouse)
Expression Region 1-747
Target Protein Sequence MDIASPPTSK CITYWKRKVK SEYMRLRQLK RLQANMGAKA LYVANFAKVQ EKTQILNEEW KKLRVQPVQP MKPVSGHPFL KKCTIESIFP GFDSQDMLMR SLNTVALVPI MYSWSPLQQN FMVEDETVLC NIPYMGDEVK EEDETFIEEL INNYDGKVHG EEEMIPGSVL ISDAVFLELV DALNQYSDEE EDGHNDPSDG KQDDSKEDLP VTRKRKRHAI EGNKKSSKKQ FPNDMIFSAI ASMFPENGVP DDMKERYREL TEMSDPNALP PQCTPNIDGP NAKSVQREQS LHSFHTLFCR RCFKYDCFLH PFHATPNVYK RKNKEIKIEP EPCGTDCFLL LEGAKEYAML HNPRSKCSGR RRRRHPVVSA SCSNASASAM AETKEGDSDR DTGNDWASSS SEANSRCQTP TKQKASPAPA QLCVVEAPSE PVEWTGAEES LFRVFHGTYF NNFCSIARLL GTKTCKQVFQ FAVKESLILK LPTDELMNPA QKKKRKHRLW AAHCRKIQLK KDNNSTQVYN YQPCDHPDRP CDSTCPCIMT QNFCEKFCQC SPDCQNRFPG CRCKTQCNTK QCPCYLAVRE CDPDLCLTCG ASEHWDCKVV SCKNCSIQRG LKKHLLLAPS DVAGWGTFIK ESVQKNEFIS EYCGELISQD EADRRGKVYD KYMSSFLFNL NNDFVVDATR KGNKIRFANH SVNPNCYAKV VMVNGDHRIG IFAKRAIQAG EELFFDYRYS QADALKYVGI ERETDVF
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Gene References into Functions
  1. The combined loss of Ezh1 and Ezh2 in chondrocytes severely impairs skeletal growth in mice. PMID: 27897169
  2. identification of EZH1 as a repressor of haematopoietic multipotency in the early mammalian embryo PMID: 29342143
  3. Suggest that EZH1 and -2 are novel targets of miR-214-3p, and miR-214-3p might be one potential miRNA for the prevention of cardiac fibrosis. PMID: 27823969
  4. Ezh1 is required for neonatal heart regeneration and development. PMID: 28512107
  5. the expression level of Ezh1 determines the restoration of H3K27 methylation in the absence of the canonical EZH2-PRC2. PMID: 28254491
  6. These results clearly demonstrated an essential role of Ezh1 in the pathogenesis of hematopoietic malignancies induced by Ezh2 insufficiency, and highlighted the differential functions of Ezh1 and Ezh2 in hematopoiesis. PMID: 26219303
  7. Loss of EZH1 is associated with increased liver injury and a blunted regenerative response. PMID: 25477280
  8. Data show that histone lysine methyltransferase Ezh1 promotes toll-like receptor (TLR)-triggered inflammatory cytokine production by suppressing the Toll-interacting protein (Tollip), contributing to full activation of the innate immunity. PMID: 25687760
  9. presence of Ezh1 helps to maintain PRC2 occupancy on its target genes in myoblasts where Jarid2 is not expressed. PMID: 24352422
  10. Deletion of Ezh1 and Ezh2 from skin results in a marked change in fate determination in epidermal progenitor cells, leading to an increase in the number of lineage-committed Merkel cells, specialized subtype of skin cells involved in mechanotransduction. PMID: 23673358
  11. Ezh1 is an important histone methyltransferase for hematopietic stem cell maintenance. PMID: 23122289
  12. Ezh1 has a role in promoting mRNA transcription. PMID: 22196887
  13. while Ezh1/2-null hair follicles (HFs) arrest morphogenesis and degenerate due to defective proliferation and increased apoptosis, epidermis hyperproliferates and survives engraftment PMID: 21317239

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Subcellular Location Nucleus
Protein Families Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily
Tissue Specificity Expressed at high levels in kidney, adrenal gland, testis and brain.
Database Links

KEGG: mmu:14055

STRING: 10090.ENSMUSP00000102906

UniGene: Mm.5027

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