Recombinant Mouse Homeobox protein NOBOX (Nobox)

Code CSB-YP855365MO
MSDS
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Source Yeast
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Code CSB-EP855365MO
MSDS
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Source E.coli
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Code CSB-EP855365MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855365MO
MSDS
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Source Baculovirus
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Code CSB-MP855365MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nobox
Uniprot No.
Alternative Names
Nobox; Og2xHomeobox protein NOBOX; Homeodomain-containing protein OG-2; Newborn ovary homeobox protein; Oocyte-specific homeobox protein
Species
Mus musculus (Mouse)
Expression Region
1-527
Target Protein Sequence
MEPTEKLCKK MQGQEAGDKP RTAALETEGP LQDSALPIQD DQDKQSSLPR ASLGKRPLSK TSEELMDAGT CRVHKAPTAA ACGPQSEEEG CSPPERKAES LKPSISAVPG QATAGSLNSH EGDLKKESLE VTCQFRKKTR TLYRSDQLEE LERIFQEDHY PDSDKRHEIS QMVGVTPQRI MVWFQNRRAK WRKVEKLNEK ETKNGPAAPS ADSSQHRSAP ELLDPMPTDL EPGPVPPENI LDVFPEPPML LTSEQTLTPF QNNEGAERVA VTPPLLSPPP IRRANLPLPL GPVQTPQVLP PMRDVPGSDS IYKDKAYVSW GTSIASPPTY SNLEDLGSQD YQASSQLGSF QLSQAPHLPL FPSLQSQFPY LPPFPYPIPS SMPFLPPEDS LFSFPFGFSG DSSQDYCPGP PPGQILLQPP AENMGTGPWS GHCLPEPPFP RPHYPQALGQ PLGAEGYFPN LLPTPYALTM SKQSSLGLNG LLEGTRVETG SSLSKMSDEQ TSSSLEQPAL EEVRDKNKNS HAAGAKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor which plays an essential role in postnatal follicle development. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly regulates the transcription of POU5F1 and GDF9 during early folliculogenesis.
Gene References into Functions
  1. The NOBOX protein is expressed throughout oocyte growth in the nucleus of ovarian Non Surrounded Nucleolus and in MII(Non Surrounded Nucleolus) oocytes; beginning with Surrounded Nucleolus oocytes of 61-70 microm, it becomes almost undetectable. PMID: 23585350
  2. These results indicate that premature ovarian failure syndrome in Nobox deficient mice results from the faulty signaling between somatic and germ line components during embryonic development. PMID: 21369782
  3. identified one Nobox DNA-binding element within the Pad6 promoter. findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis. PMID: 20659469
  4. Nobox is critical for specifying an oocyte-restricted gene expression pattern essential for postnatal follicle development PMID: 15326356
  5. NOBOX directly regulates the transcription of Pou5f1 and Gdf9 in oocytes during early folliculogenesis PMID: 16997917
  6. findings indicate that Nobox is likely an activator of oocyte-specific gene expression PMID: 17494914
  7. RT-PCR data shows an increase in oogenesis specific gene transcripts (Nobox, Oct4, Bmp15, Gdf9, Oogenesin1 and Oogenesin2) between the primordial until the preantral stages, with the exception of the Oogenesin1 transcripts under gonadotropin-induction. PMID: 19480014

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Subcellular Location
Nucleus.
Tissue Specificity
Specifically expressed in ovaries and testes. In ovaries, expressed in oocytes from primordial through antral follicles but not in granulosa cells, theca cells and corpora lutea.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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