Recombinant Mouse Immunity-related GTPase family M protein 1 (Irgm1)

Code CSB-YP730655MO
MSDS
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Source Yeast
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Code CSB-EP730655MO
MSDS
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Source E.coli
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Code CSB-EP730655MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730655MO
MSDS
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Source Baculovirus
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Code CSB-MP730655MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Irgm1
Uniprot No.
Alternative Names
Irgm1; Ifi1; Iigp3; IrgmImmunity-related GTPase family M protein 1; EC 3.6.5.-; Interferon-inducible GTPase 3; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47; LRG-47
Species
Mus musculus (Mouse)
Expression Region
1-409
Target Protein Sequence
MKPSHSSCEA APLLPNMAET HYAPLSSAFP FVTSYQTGSS RLPEVSRSTE RALREGKLLE LVYGIKETVA TLSQIPVSIF VTGDSGNGMS SFINALRVIG HDEDASAPTG VVRTTKTRTE YSSSHFPNVV LWDLPGLGAT AQTVEDYVEE MKFSTCDLFI IIASEQFSSN HVKLSKIIQS MGKRFYIVWT KLDRDLSTSV LSEVRLLQNI QENIRENLQK EKVKYPPVFL VSSLDPLLYD FPKLRDTLHK DLSNIRCCEP LKTLYGTYEK IVGDKVAVWK QRIANESLKN SLGVRDDDNM GECLKVYRLI FGVDDESVQQ VAQSMGTVVM EYKDNMKSQN FYTLRREDWK LRLMTCAIVN AFFRLLRFLP CVCCCLRRLR HKRMLFLVAQ DTKNILEKIL RDSIFPPQI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Putative GTPase which is required for IFNG-mediated clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. Required for macrophage motility and possibly also for adhesion.
Gene References into Functions
  1. This work establishes the importance of Irgm1(-/-) mice as a model to elucidate host-environment interactions that regulate mucosal homeostasis and intestinal inflammatory responses. PMID: 29361512
  2. Irgm1/IRGM participates in the polarization of M1 macrophages, is upregulated in human atheromata, and promotes development of atheroma in murine experimental atherosclerosis PMID: 27439214
  3. M1 macrophage may promote Th1/Th17 cell differentiation during the early experimental autoimmune encephalomyelitis, and the proinflammatory function of M1 cells requires Irgm1. PMID: 27443879
  4. A series of metabolic studies indicated that the enhanced cytokine production was associated with marked metabolic changes in the Irgm1-deficient macrophages, including increased glycolysis and an accumulation of long chain acylcarnitines. PMID: 28154172
  5. data suggested that the deficiency of LRG-47 might affect host's CD4(+) T cell immune response via the weakening of IFN-gamma downstream signaling PMID: 26660918
  6. Irgm1-/- embryonic fibroblasts show autophagic flux impairment and defective lysosomal degradation. PMID: 27098192
  7. Interestingly, we observed that IRGM1 enhanced F-actin polymerization and triggers epithelial mesenchymal transition (EMT) through a mechanism involved in PIK3CA mediated Rac1 activation. PMID: 26202910
  8. Interferon-beta activation recruited the immunity-related GTPase M1 (IRGM1) to the Toxoplasma gondii-occupied parasitophorous vacuole in peritoneal macrophages and murine embryonic fibroblasts. PMID: 25628099
  9. these data reveal an Irgm1-dependent mechanism that promotes the tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy PMID: 25619828
  10. Palmitoylation together with the alphaK helix firmly anchor Irgm1 in the Golgi and mitochondria, thus facilitating function of the protein. PMID: 24751652
  11. 2 Irgm1 isoforms generated by differential splicing localize to the Golgi apparatus & weakly to mitochondria. Only the long isoforms is seen on endolysosomal membranes. Irgm1 is a negative regulator of the immunity-related GTPase protein family. PMID: 23842753
  12. the paralogous IRGM proteins Irgm1 and Irgm3 fail to robustly associate with "non-self" PVs containing either the bacterial pathogen Chlamydia trachomatis or the protozoan pathogen Toxoplasma gondii PMID: 23785284
  13. By 9 weeks post-infection, fewer Brucella abortus were recovered from LRG-47(-/-) mice than controls, suggesting that the host protein has a positive role in maintaining long term persistence of the bacteria within the host. PMID: 22919646
  14. These results suggest that Irgm1 modulates acute inflammatory responses in the mouse intestine, putatively through the regulation of gut autophagic processes, that may be pivotal for proper Paneth cell functioning. PMID: 23989005
  15. Loss of IRGM1 is associated with impaired CD36 internalization in macrophage, which was associated with the deficiency of F-actin polymerization in atherosclerosis. PMID: 23689639
  16. These data demonstrate a novel role of IRGM1 in regulating neuronal autophagy and survival during ischemic stroke. PMID: 22874556
  17. These data suggest that Irgm1 is a proinflammatory factor for Experimental autoimmune encephalomyelitis PMID: 22796503
  18. IFN-gamma enhances Con A-induced autophagic flux and causes an IRGM1-dependent lysosome-mediated necrotic cell death in hepatocytes PMID: 22163006
  19. Data show that hyperproliferation, self-renewal, and autophagy defects in Irgm1(-/-) HSCs were normalized in double-knockout Irgm1(-/-)Ifngr1(-/-) and Irgm1(-/-)Stat1(-/-), and the defects were abolished in Irgm1(-/-)Irgm3(-/-) double-knockout. PMID: 21633090
  20. Irgm1/LRG-47 is a significant factor in the progression of experimental autoimmune encephalomyelitis and multiple sclerosis. PMID: 20056715
  21. tagging of Irgm1 leads to loss of Golgi localisation and enhanced localisation on endolysosomal membranes, probably as a result of constitutive activation PMID: 20072621
  22. Irgm1 likely controls macrophage motility by regulating the positioning of specific GKS IRG proteins to the plasma membrane, which in turn, modulate cytoskeletal remodeling PMID: 19920210
  23. LRG-47 may serve as a critical vacuolar trafficking component used to dispose of intracellular pathogens like Mycobacterium tuberculosis PMID: 14576437
  24. A role is established for LRG-47 in host control of mycobacteria; in the context of the IFN-gamma response to persistent infection, LRG-47 can have downstream regulatory effects on lymphocyte survival. PMID: 14707092
  25. LRG-47 is a Golgi-associated protein in the IFN-stimulated cell, which is rapidly recruited to active plasma membrane upon phagocytosis and remains associated with phagosomes as they mature PMID: 15294976
  26. LRG-47 can influence pathogen control by simultaneously regulating macrophage-microbicidal activity and hemopoietic function PMID: 16339555
  27. Irgm1 (LRG-47)induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium var. bovis BCG PMID: 16888103
  28. p47 GTPase LRG47 is involved in resistance to Trypanosoma cruzi infection in dendritic cells and macrophages. PMID: 17082622
  29. In lipopolysaccharide-stimulated murine macrophages LRG47 is induced by IFN-beta and negatively regulates Toll-like receptor 4 signaling to prevent excess proinflammatory cytokine production and shock. PMID: 17911638
  30. Irgm1 plays a key role in regulating the adhesion and motility of activated macrophages. PMID: 17982087
  31. inhibits baseline hematopoietic stem cells (HSC) proliferation and is required for a normal HSC response to chemical and infectious stimuli PMID: 18371424
  32. Irgm1 is essential for the expansion of CD4+ T lymphocyte populations after TCR stimulation. PMID: 18806793
  33. Irgm1 plays a major role in maintaining T lymphocyte homeostasis during host IFN gamma responses by protecting these cells from autophagy-dependent cell death. PMID: 19066452
  34. involved in interferon-gamma dependent control of M. tuberculosis infection in mouse model of tuberculosis PMID: 19120485
  35. Irgm3 functions to counter the effects of Irgm1. PMID: 19176402
  36. Cooperation between Irgm1 and PI(3)K further facilitated the engagement of Irgm1 with its fusogenic effectors at the site of infection, thereby ensuring pathogen-directed responses during innate immunity. PMID: 19620982

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Subcellular Location
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasmic vesicle, autophagosome membrane. Lysosome membrane. Late endosome membrane. Cell projection, phagocytic cup. Note=Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing phagosomes. Association with phagosomes is dependent on nucleotide-binding but is IFNG-independent. Preferentially associated with cis- and medial-Golgi. Also detected in late endosomes and lysosomes.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, IRG family
Tissue Specificity
Expressed in lung and primary macrophages.
Database Links
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