Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Itgax; Integrin alpha-X; CD11 antigen-like family member C; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD antigen CD11c
Species
Mus musculus (Mouse)
Expression Region
20-1116aa
Target Protein Sequence
FNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITELFTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGKAFYNEHSKQELKAIASMPSHEYVFSVENFDALKDIENQLKEKIFAIEGTETPSSSTFELEMSQEGFSAVFTPDGPVLGAVGSFSWSGGAFLYPSNMRPTFINMSQENEDMRDAYLGYSTALAFWKGVHSLILGAPRHQHTGKVVIFTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIGVPHYYEHTRGGQVSVCPMPGVGSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADVAIGAPGEEENRGAVYIFHGASRQDIAPSPSQRISASQIPSRIQYFGQSLSGGQDLTRDGLVDLAVGSKGRVLLLRTRPILRVSPTVHFTPAEISRSVFECQEQVAPEQTLSDATVCLHIHESPKTQLGDLRSTVTFDLALDHGRLSTRAIFKETKTRALTRVKTLGLNKHCESVKLLLPACVEDSVTPITLRLNFSLVGVPISSLQNLQPMLAVDDQTYFTASLPFEKNCGADHICQDDLSVVFGFPDLKTLVVGSDLELNVDVTVSNDGEDSYGTTVTLFYPVGLSFRRVAEGQVFLRKKEDQQWQRRGQHSLHLMCDSTPDRSQGLWSTSCSSRHVIFRGGSQMTFLVTFDVSPKAELGDRLLLRARVGSENNVPGTPKTTFQLELPVKYAVYTMISSHDQFTKYLNFSTSEKEKTSVVEHRFQVNNLGQRDVPVSINFWVPIELKGEAVWTVMVSHPQNPLTQCYRNRLKPTQFDLLTHMQKSPVLDCSIADCLHLRCDIPSLGILDELYFILKGNLSFGWISQTLQKKVLLLSEAEITFNTSVYSQLPGQEAFLRAQTKTVLEMYKVHNPVP
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal MBP-tagged and C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Insertion of the gene encoding the Mouse Itgax protein (20-1116aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal MBP tag and C-terminal 6xHis tag is attached to the protein. Following expression, the recombinant Mouse Itgax protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Mouse Itgax protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.
