Recombinant Mouse Interferon regulatory factor 2 (Irf2)

Code CSB-YP011815MO
MSDS
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Source Yeast
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Code CSB-EP011815MO
MSDS
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Source E.coli
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Code CSB-EP011815MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011815MO
MSDS
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Source Baculovirus
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Code CSB-MP011815MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Irf2
Uniprot No.
Alternative Names
Irf2; Interferon regulatory factor 2; IRF-2
Species
Mus musculus (Mouse)
Expression Region
1-349
Target Protein Sequence
MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI HTGKHQPGID KPDPKTWKAN FRCAMNSLPD IEEVKDRSIK KGNNAFRVYR MLPLSERPSK KGKKPKTEKE ERVKHIKQEP VESSLGLSNG VSGFSPEYAV LTSAIKNEVD STVNIIVVGQ SHLDSNIEDQ EIVTNPPDIC QVVEVTTESD DQPVSMSELY PLQISPVSSY AESETTDSVA SDEENAEGRP HWRKRSIEGK QYLSNMGTRN TYLLPSMATF VTSNKPDLQV TIKEDSCPMP YNSSWPPFTD LPLPAPVTPT PSSSRPDRET RASVIKKTSD ITQARVKSC
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Gene References into Functions
  1. Mysm1 enhanced function of the IRF2 and IRF8 promoters, suggesting that Mysm1 governs the IRFs for hematopoietic stem cell homeostasis. PMID: 27277682
  2. Interferon regulatory factor 2 protects mice from lethal viral neuroinvasion PMID: 27899441
  3. Chimeric IRF-2 binds with IRF-E motif and VREbeta but not with VREalpha1. PMID: 25251598
  4. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
  5. These results revealed a multi-faceted role of IRF-2 in the function of B cells, particularly B2 cells, through regulating proliferation in an IFNAR-dependent manner and antibody production via up-regulation of Blimp-1. PMID: 22773153
  6. In mice doubly deficient forIRF-2 and CD1d, the overall memory phenotype T cell population is contrastingly enlarged. PMID: 22504642
  7. IRF2 regulates exocytosis in pancreatic acinar cells; defects in this process might be involved in the early phases of acute pancreatitis. PMID: 21699790
  8. Forced expression of the interferon regulatory factor 2 oncoprotein causes polyploidy and cell death in FDC-P1 myeloid hematopoietic progenitor cells. PMID: 11980642
  9. Irf2 has an important role in vitro in the regulation of COX-2 expression by LPS. PMID: 12537697
  10. IRF-2 is acetylated in a cell growth-dependent manner, which enables it to contribute to transcription of cell growth-regulated promoters PMID: 12738767
  11. IRF-2 is a critical transacting factor that regulates in vivo responses to lipopolysaccharide, with a novel anti-apoptotic role. PMID: 12759457
  12. In the absence of IRF-2, uterine natural killer (uNK) cells are normal in number and function, but there is a serious deficiency of peripheral NK cells. PMID: 12960317
  13. IFN regulatory factor 2 is involved in the negative regulation of IFN-alpha/beta signaling. PMID: 14983024
  14. Binding affinity of IRF-2 is similar to that of IRF-1 and B lymphocyte-induced maturation protein-1 (Blimp-1) for functionally important regulatory sites containing the consensus sequence GAAAG. PMID: 15494505
  15. IRF-2 is involved in up-regulation of nonmuscle myosin heavy chain II-A gene expression in cell differentiation PMID: 15496418
  16. Differential peripheral and central natural killer (NK) cell deficiencies in IRF-2 knockout mice reveal a novel late checkpoint for NK cell maturation, distinct from the early IL-15-dependent expansion stage. PMID: 15879093
  17. the negative regulatory role of IRF-2 on the basophil population size is critically important for preventing excess Th2 polarization and the Th1/Th2 balance in naive animals PMID: 15914553
  18. Together, these data demonstrate that IRF-2 is involved in the regulation of TR2 expression in EL-4 T cells. PMID: 18155158
  19. Irf2-/- HSCs showed enhanced cell cycling status and failed to produce hematopoietic cells in competitive repopulation assays PMID: 19483695
  20. IRF-2 plays an important role in megakaryopoiesis in inflammatory states. PMID: 19818776

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Subcellular Location
Nucleus.
Protein Families
IRF family
Database Links
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