Recombinant Mouse Intraflagellar transport protein 74 homolog (Ift74)

Code CSB-YP805913MO
MSDS
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Source Yeast
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Code CSB-EP805913MO
MSDS
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Source E.coli
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Code CSB-EP805913MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805913MO
MSDS
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Source Baculovirus
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Code CSB-MP805913MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ift74
Uniprot No.
Alternative Names
Ift74; Ccdc2; Cmg1; Intraflagellar transport protein 74 homolog; Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2
Species
Mus musculus (Mouse)
Expression Region
1-600
Target Protein Sequence
MASNHKSSAP RPISRGGIGL TGRPPSGIRP PSGNVRVATA MPPTTARPGS RGGPLGTGGV LSSQIKVADR PVTQQGLSGM KTGMKGPQRQ ILDKSYYLGL LRSKISELTT EINKLQKEIE MYNQENSVYL SYEKRAETLA VEIKDFQGQL ADYNMLVDKL NTNTEMEEVM SDYNMLKAQN DRETQSMDVI FTERQAKEKQ IRSVEEEVEQ EKQAADGIIK NMSPEKQVKY IEMKTTNEKL LQELDTLQQQ LDSLNMKKES LETEIAHSQV KQEAVLLYEK LYELESHRDQ MIAEDKSMGS PMEERERLLK QVKEDNQEIA SMERQLTDIK EKINQFSEEI RQLDMDLEEH QGEMNQKYKE LKKREENMDA FIETFEETKN QELERKAQIE ASIITLLEHC SRNINRMKQI SSITNQELKM MQDDLSFKST EMQKSQTTAR NLTSDSQRLQ LDLQKMELLE SKMTEEQQSL KNKIKQMTAD LETYSDLAAL KSSAEEKKKK LHQERTVLST HRNAFKKIME KLTSDYDTLK RQLQDNETHA QLTNLERKWQ HLEQNNFVMK EFIATKSQES DYQPVIKNVM KQIAEYNKTI MDALHNASRS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis.
Gene References into Functions
  1. The CMG-1 serves as a transcriptional regulator of proliferation and adhesion-associated genes in early stage male germ-line cells in the testis. PMID: 20545763
  2. Results identify a cDNA encoding the mouse ortholog of capillary morphogenesis gene (CMG)-1, and suggest that CMG-1 may play a role in the transcriptional regulation of the cyclin D2 gene. PMID: 16705683
  3. Ift74 is most abundantly expressed in tissues rich in highly ciliated cells, such as olfactory sensory neurons, and is predicted to be important to cilia. PMID: 17971504
Subcellular Location
Cell projection, cilium. Cytoplasmic vesicle.
Protein Families
IFT74 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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