Recombinant Mouse Kinesin-like protein KIF11 (Kif11), partial

Code CSB-YP747439MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP747439MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP747439MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP747439MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP747439MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Kif11
Uniprot No.
Alternative Names
Kif11; Kinesin-like protein KIF11; Kinesin-related motor protein Eg5
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Gene References into Functions
  1. NEK7 regulates dendrite morphogenesis in vitro and in vivo. NEK7 kinase activity is required for dendrite growth and branching, as well as spine formation and morphology. NEK7 regulates these processes in part through phosphorylation of the kinesin Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites. PMID: 29899413
  2. that Pten, through the Dlg1-binding ability of its PDZ-binding domain , accumulates phosphorylated Eg5 at duplicated centrosomes to establish symmetrical bipolar spindles that properly segregate chromosomes PMID: 27240320
  3. Fbxo30-Eg5 interaction as a critical checkpoint in mammopoiesis. PMID: 27117404
  4. Data show that the STIM1 Ksp (Eg5)-cre knockout mice produced more urine compared to control. PMID: 27336410
  5. Data suggest that amyloid-beta (here, Abeta 1-42) induces acute and chronic synaptic dysfunction in part through inhibition of Eg5 (kinesin-5) in hippocampal neurons. PMID: 26957206
  6. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes beta-actin mRNA and affects cell migration. PMID: 25588836
  7. Its inhibition reduces neutrotrophin and transmitter receptor function. PMID: 24636920
  8. These results suggest that 4-(N-(2-(N-acetylcysteine-S-yl) acetyl) amino)-4'- (N-(2-(N-(triphenylmethyl)amino)acetyl)amino)azobenzene could be used as photochromic inhibitor of Eg5 to achieve photocontrol of living cells. PMID: 24451491
  9. incorporation of photochromic molecules into the key region of loop L5 facilitates the photocontrol of the function of kinesin Eg5. PMID: 24334276
  10. Finally, when kinesin spindle protein (KSP) siRNA was encapsulated in lipid nanoparticles containing a modest amount of PEG, the proliferation of endothelial cells was inhibited due to the efficient knock down of KSP mRNA. PMID: 24607899
  11. These results show that intratumoral electro-transfer of siRNA is feasible and kinesin spindle protein (KSP)-specific siRNA may provide a novel strategy for therapeutic intervention. PMID: 22552964
  12. observations explain the poleward accumulation of Eg5 in early mitosis and its redistribution in anaphase. Inhibition of dynein blocked Eg5 movement on microtubules, whereas depletion of the Eg5-binding protein TPX2 resulted in end-directed Eg5 movement. PMID: 22337772
  13. Observations further support the conclusion that Eg5 is essential for cell division early in mouse development. PMID: 17449012
  14. Results indicate that Eg5 overexpression disrupts the unique balance of forces associated with normal spindle assembly and function, and thereby leads to the development of spindle defects, genetic instability, and tumors. PMID: 17974955
  15. Eg5 is essential during early mouse development and cannot be compensated by another molecular motor. PMID: 18474226

Show More

Hide All

Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1