Recombinant Mouse Kinesin-like protein KIF2C (Kif2c)

Code CSB-YP849733MO
MSDS
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Source Yeast
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Code CSB-EP849733MO
MSDS
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Source E.coli
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Code CSB-EP849733MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP849733MO
MSDS
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Source Baculovirus
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Code CSB-MP849733MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Kif2c
Uniprot No.
Alternative Names
Kif2cKinesin-like protein KIF2C; Mitotic centromere-associated kinesin; MCAK
Species
Mus musculus (Mouse)
Expression Region
1-721
Target Protein Sequence
MESLHARLFP GLSINIQRSN GLIHPANIST VNVEKSCVSV EWIEGGTTKG KEIDIDDVAA INPELLQLLP LRPKDSLPLQ ENVTVPKQKR KSVNSKIPAL KEGLRSRSTR MSTVSEVRIP AQENEMEVEL PVPTNSRKQF AIPSHPRASC STVTELPLLM VSEEAEEQAH STRSTSSANP GNSVRRKSCI VKEMEKMKNK REEKRAQNSE LRIKRAQEYD SSFPNWEFAR MIKEFRVTME CSPLTVTDPI EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP KLKVDLTKYL ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRNLN LEVYVTFFEI YNGKVFDLLN KKAKLRVLED SRQQVQVVGL QEYLVTCADD VIKMINMGSA CRTSGQTFAN SNSSRSHACF QILLRTKGRL HGKFSLVDLA GNERGADTSS ADRQTRMEGA EINKSLLALK ECIRALGQNK AHTPFRESKL TQVLRDSFIG ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP SGEQPVQMET EVMEASSNGT SLTGNEEEEL SSQMSSFNEA MTQIRELEER ALEELREIIQ QGPNWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALREVIKAL RLAMQLEEQA SKQINSKKRH Q
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment.
Gene References into Functions
  1. PAK1 phosphorylates MCAK and regulates both its localization and function. PMID: 23055517
  2. MCAK appears to possess a unique distribution and function in oocyte maturation. PMID: 20406800
  3. MCAK contributes to chromosome alignment in meiosis I, but is not necessary for preventing chromosome segregation errors. PMID: 20504960
  4. Possible functions of MCAK at the inner centromere domain and at the perikinetochoric ring during both meiotic divisions. PMID: 16741559
Subcellular Location
Cytoplasm, cytoskeleton. Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, MCAK/KIF2 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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