Recombinant Mouse Macrophage asialoglycoprotein-binding protein 1 (Mgl1), partial

Code CSB-YP342996MO
MSDS
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Source Yeast
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Code CSB-EP342996MO
MSDS
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Source E.coli
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Code CSB-EP342996MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342996MO
MSDS
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Source Baculovirus
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Code CSB-MP342996MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Clec10a
Uniprot No.
Alternative Names
Clec10a; Mgl; Mgl1C-type lectin domain family 10 member A; MMGL; Macrophage asialoglycoprotein-binding protein 1; M-ASGP-BP-1; Macrophage galactose/N-acetylgalactosamine-specific lectin
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Recognizes terminal galactose and N-acetylgalactosamine units. May participate in the interaction between tumoricidal macrophages and tumor cells. Plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity.
Gene References into Functions
  1. Macrophage Galactose-Type Lectin-1 Deficiency Is Associated with Increased Neutrophilia and Hyperinflammation in Gram-Negative Pneumonia PMID: 26912318
  2. Mgl1-deficient mice displayed accelerated colonic propulsion and were hypersensitive to mu receptor-mediated inhibition of colonic motility. PMID: 26075589
  3. MGL1 is involved in T. crassiceps recognition and subsequent innate immune activation and resistance. PMID: 25664320
  4. The MGL1 functions as an attachment and entry receptor for influenza A virus. PMID: 24257596
  5. Syk was involved in the CLR-mediated activation of mast cells by BP PMID: 23794711
  6. Hoxc8 downregulates Mgl1 tumor suppressor gene expression and reduces its concomitant function on cell adhesion. PMID: 21773674
  7. The authors conclude that lectin-mediated interactions of influenza virus with the MMR or the MGL are required for the endocytic uptake of the virus into macrophages. PMID: 20106926
  8. mMGL1 preferentially binds Lewis X-conjugated soluble polyacrylamides PMID: 12016228
  9. Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins. PMID: 12077344
  10. sialoadhesin acts as an endogenous counter-receptor for MGL1 PMID: 15364954
  11. The mMGL1 protein is induced in diverse populations of aaMF, including peritoneal macrophages elicited during infection with the protozoan Trypanosoma brucei brucei or the Helminth Taenia crassiceps and alveolar macrophages elicited. PMID: 15591125
  12. MGL1 expressed on a specific subpopulation of macrophages that secrete IL-1alpha was proposed to regulate specific cellular interactions crucial to granulation tissue formation PMID: 15784728
  13. MGL1/CD301a is involved in the clearance of apoptotic cells PMID: 16096344
  14. analysis of protein-protein interactions in the Mgl-1 oncoprotein PMID: 16969496
  15. MGL1 mutants of Ala89Arg and Ala89Arg/Pro115Arg showed beta-GalNAc bindings. PMID: 18053814
  16. Carbohydrate binding mechanism of the macrophage galactose-type C-type lectin 1 revealed by saturation transfer experiments.( PMID: 18790731
  17. MGL1/CD301a plays a protective role against colitis PMID: 19095961
  18. MGL1 as a novel regulator of inflammatory monocyte trafficking to adipose tissue in response to diet-induced obesity. PMID: 19995956

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Subcellular Location
Membrane; Single-pass type II membrane protein.
Tissue Specificity
Detected in lymph node in the subcapsular sinus, interfollicular regions, T and B-cell boundary and in the areas surrounding high endothelial venules (at protein level). Expressed on the surface of activated macrophages. Expressed in heart, lung, testis,
Database Links
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