Recombinant Mouse N-terminal EF-hand calcium-binding protein 3 (Necab3)

Code CSB-YP863526MO
MSDS
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Source Yeast
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Code CSB-EP863526MO
MSDS
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Source E.coli
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Code CSB-EP863526MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863526MO
MSDS
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Source Baculovirus
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Code CSB-MP863526MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Necab3
Uniprot No.
Alternative Names
Necab3; Apba2bp; Xb51; N-terminal EF-hand calcium-binding protein 3; Amyloid-beta A4 protein-binding family A member 2-binding protein; X11L-binding protein 51; mXB51
Species
Mus musculus (Mouse)
Expression Region
1-353
Target Protein Sequence
MACAGLLTVC LLGPPAPQQP RHSAPAAGHA LFQDVFRRAD KNDDGKLSFE EFQNYFADGV LSSAELRELF SGIDDHLTDN LETEKLCDYF STHLGVYRPV LAALESLNRA VLTAMDTTKL EYEQASKVDQ FVTRFLLRET VNQLQALQTS LEGASDTLEA QAHGQRLDEE TIKAQSRPCG SRRAGRRALR SISWSPSWSP GSSDTGRSSE AEQQWRLQVN RLQELIDQLE CKAPRLEPTH EEDLTKGFDS HILVAQRQVQ VAEDALQDFH RALCCYMNFT GAQSHCLHVS AQKMLDNAAF TLYEFWQDEA SWRRHQQSPC SKAFQRTLID HLQAPDTLTT VFFPASWWIM NNN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3.
Gene References into Functions
  1. The genes for the three Necab's, including Necab-3, were expressed in specific, non-overlapping areas of the hippocampus. PMID: 23850650
  2. Physical interactions of Nek2 with NIP1 were confirmed. In fact, Nek2 can phosphorylate NIP1 in vivo. Immunostaining experiments revealed that NIP1 is a Golgi protein. PMID: 14697346
  3. xb51 may be required for maturation and maintenance of xb51-expressing neurons in the forebrain PMID: 19035353
Subcellular Location
Golgi apparatus.
Tissue Specificity
Widely expressed, with highest levels in the brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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