Recombinant Mouse Nicotinate phosphoribosyltransferase (Naprt1)

Code CSB-YP807484MO
MSDS
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Source Yeast
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Code CSB-EP807484MO
MSDS
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Source E.coli
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Code CSB-EP807484MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP807484MO
MSDS
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Source Baculovirus
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Code CSB-MP807484MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Naprt
Uniprot No.
Alternative Names
Naprt; Naprt1; Nicotinate phosphoribosyltransferase; NAPRTase; EC 6.3.4.21; Nicotinate phosphoribosyltransferase domain-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-538
Target Protein Sequence
MEMELDSEGR MVVRPLLTDL YQATMALGYW RAGRACEAAE FELFFRHCPF GGSFALSAGL QDCMRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD CSGVTVRALP EGSLAFPGVP LLQVSGPLLL VQLLETPLLC LVSYASLVAT NAARLRLIAG PDKKLLEMGL RRAQGPDGGF TASIYSYLGG FDSSSNTLAG QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APASSEGPTV DLPARVNLWL KRVCLYLGLE EQEPHPGERA AFVAYALAFP RAFQGLLDSY SVRRSGLPNF LAVALALGEL GYRAVGVRLD SGDLLQQAKE IRGIFRTAGA QFQMPWLESV PIAVSNNIDE SELMRLAQKG SEVNVIGIGT SVVTCPKQPS MGCVYKLVSV GGQPRIKLTE DPEKQTLPGS KAAFRFLGPD GSLLLDLLQL AEEPPPKAGQ ELRVWPRGTQ EPCTVKPAQV EPLLRLYLQQ GQLCEPLPSL DESRRFAQQS LSLLRPAHKQ LQSPAVYPVA LSEKLRALVD SLSARGPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.
Gene References into Functions
  1. Synthesis and Degradation of Adenosine 5'-Tetraphosphate by Nicotinamide and Nicotinate Phosphoribosyltransferases PMID: 28416276
  2. NA elevates cellular NAD levels through NAPRT function and, thus, protects the cells against stress, partly due to lack of feedback inhibition of NAPRT but not NamPRT by NAD PMID: 17604275
Subcellular Location
Cytoplasm, cytosol.
Protein Families
NAPRTase family
Tissue Specificity
Abundantly expressed in the small intestine, liver and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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