Recombinant Mouse Non-histone chromosomal protein HMG-14 (Hmgn1)

Code CSB-YP010568MO
MSDS
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Source Yeast
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Code CSB-EP010568MO
MSDS
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Source E.coli
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Code CSB-EP010568MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010568MO
MSDS
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Source Baculovirus
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Code CSB-MP010568MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hmgn1
Uniprot No.
Alternative Names
Hmgn1; Hmg-14; Hmg14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
2-96
Target Protein Sequence
PKRKVSADG AAKAEPKRRS ARLSAKPAPA KVDAKPKKAA GKDKASDKKV QIKGKRGAKG KQADVADQQT TELPAENGET ENQSPASEEE KEAKSD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
Gene References into Functions
  1. our findings show that HMGN1 contributes to antitumor immunity and it may offer an effective adjuvant to heighten responses to cancer vaccines. PMID: 25205103
  2. in mice, loss of HMGN1 leads to transcription changes that accelerate the progression of DEN-induced hepatocarcinogenesis, without affecting the type of tumors or the final total tumor burden of these mice. PMID: 24296759
  3. HMGN1 is a nucleosome remodeling protein encoded on chromosome 21q22 that suppresses H3K27me3 and promotes both B cell proliferation in vitro and B-ALL in vivo PMID: 24747640
  4. Phenotypic analysis of Hmgn1(tm1/tm1), Hmgn3(tm1/tm1), and Hmgn5(tm1/tm1) mice and their wild type littermates with a battery of standardized tests uncovered variant-specific abnormalities. PMID: 23620591
  5. a regulatory role for HMGN1 in PARP-1 activation. PMID: 22736760
  6. extracellular HMGN1 acts as a novel alarmin critical for LPS-induced development of innate and adaptive immune responses. PMID: 22184635
  7. HMGN1 regulates the expression of methyl CpG-binding protein 2 (MECP2) in mouse and human, and affects the behavior of mice PMID: 22009741
  8. Data show that HMGN1, a nuclear protein that binds to nucleosomes and reduces the compaction of the chromatin fiber, modulates histone posttranslational modifications. PMID: 15327773
  9. HMGN1, a nucleosomal binding protein that reduces the compaction of the chromatin fiber, increases the levels of acetylation of K14 in H3 PMID: 16096646
  10. Hmgn1 modulates the expression of cadherins. PMID: 16279949
  11. HMGN1 plays role in chromatin remodeling during embryogenesis and in the activation of Sox9 during chondrogenesis. PMID: 16382150
  12. HMGN1 levels naturally decrease as a function of contact-mediated cell cycle arrest. PMID: 16451822
  13. HMGN1 enhances the rate of heat shock-induced H3K14 acetylation in the Hsp70 promoter PMID: 18218636
  14. Thus, by regulating the levels of histone modifications, HMGN1 affects ATM activation. PMID: 19079244
  15. Results show that the appearance of the skin and hair follicle of Hmgn1(-/-) mice was indistinguishable from that of their Hmgn1(+/+) littermates. PMID: 19303948

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
HMGN family
Database Links
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