Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nupr1

Uniprot No.

Q9WTK0

Alternative Names

Nupr1; Nuclear protein 1; Protein p8

Species

Mus musculus (Mouse)

Expression Region

1-80

Target Protein Sequence

MATLPPTANP SQQPLNLEDE DGILDEYDQY SLAHPCVVGG GRKGRTKREA AANTNRPSPG GHERKLLTKF QNSERKKAWR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription regulator that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Thereby participates in regulation of many process namely cell-cycle, apoptosis, autophagy and DNA repair responses. Controls cell cycle progression and protects cells from genotoxic stress induced by doxorubicin through the complex formation with TP53 and EP300 that binds CDKN1A promoter leading to transcriptional induction of CDKN1A. Protects pancreatic cancer cells from stress-induced cell death by binding the RELB promoter and activating its transcription, leading to IER3 transactivation. Negatively regulates apoptosis through interaction with PTMA. Inhibits autophagy-induced apoptosis in cardiac cells through FOXO3 interaction, inducing cytoplasmic translocation of FOXO3 thereby preventing the FOXO3 association with the pro-autophagic BNIP3 promoter. Inhibits cell growth and facilitates programmed cell death by apoptosis after adriamycin-induced DNA damage through transactivation of TP53. Regulates methamphetamine-induced apoptosis and autophagy through DDIT3-mediated endoplasmic reticulum stress pathway. Participates in DNA repair following gamma-irradiation by facilitating DNA access of the transcription machinery through interaction with MSL1 leading to inhibition of histone H4' Lys-16' acetylation (H4K16ac). Coactivator of PAX2 transcription factor activity, both by recruiting the EP300 cofactor to increase PAX2 transcription factor activity and by binding PAXIP1 to suppress PAXIP1-induced inhibition on PAX2. Positively regulates cell cycle progression through interaction with COPS5 inducing cytoplasmic translocation of CDKN1B leading to the CDKN1B degradation. Coordinates, through its interaction with EP300, the assiociation of MYOD1, EP300 and DDX5 to the MYOG promoter, leading to inhibition of cell-cycle progression and myogenic differentiation promotion. Negatively regulates beta cell proliferation via inhibition of cell-cycle regulatory genes expression through the suppression of their promoter activities. Also required for LHB expression and ovarian maturation. Exacerbates CNS inflammation and demyelination upon cuprizone treatment.

Gene References into Functions

Nupr1 acts as a gene modifier of the effect of Kras(G12D)-induced senescence by regulating Dnmt1 expression and consequently genome-wide levels of DNA methylation. PMID: 26617245
Hepatitis B virus X (HBx) protein can modulate NUPR1 expression through the Smad4 pathway and NUPR1 has a role in hepatocellular carcinoma progression. PMID: 26392315
Data indicate that heat shock protein 70 (Hsp70) is higher in nuclear protein 1 (Nupr1+/-) haplodeficient mice, concomitant with improved insulin sensitivity. PMID: 25638293
p8 has no impact on the cellular composition of the adaptive and innate immune systems in noninflammatory conditions. However, it may limit apoptosis and maintain homeostasis of the immune reaction during acute pancreatitis. PMID: 25098696
Nupr1 aids oncogenic Kras to bypass senescence in a manner that cooperatively promotes pancreas intraepithelial neoplasia formation. PMID: 24902898
data shows that p8 deficiency increases ROS and subsequently the expression of anti-oxidative enzymes, such as HO-1, suggesting an involvement in the anti-oxidative defense. PMID: 25475530
According to Nupr1 status, KIC mice develop tumours that phenocopy human classical or QM-PDAC, respectively, and present differential drug sensitivity, thus becoming attractive models for preclinical drug trials. PMID: 24026351
Nupr1 expression is required for IL-1beta-mediated expression of MMP-13. PMID: 24497499
Inhibition of NUPR1 expression or activity has the potential to protect against the metabolic defects associated with obesity and type 2 diabetes. PMID: 23900510
a NUPR1/RELB/IER3 stress-related pathway that is required for oncogenic Kras(G12D)-dependent transformation of the pancreas. PMID: 22565310
progressive PPARalpha- and p8-mediated ER stress contribute to the hepatocarcinogenesis in ACOX1(-/-) mice PMID: 21801867
p8-deletion resulted in reduced collagen fibrosis after transverse aortic constriction, but in turn, was associated with a detrimental higher increase in autophagy. PMID: 21775709
A role for the transcriptional regulator p8 in autophagy, is demonstrated. PMID: 20181828
P8 is a growth inhibitor that facilitates apoptosis induced in fibroblasts by DNA damage PMID: 11896600
improves pancreatic resistance to inducers of acute pancreatitis by a mechanism implicating the expression of the anti-inflammatory protein PAP I PMID: 14660681
Maintenance of the transformed phenotype of pituitary GH3 cells requires expression of p8 and that it may play a similar role when reexpressed in a subset of lactotropes that form prolactinomas in vivo. PMID: 15243129
p8 is an excellent candidate as a novel biomarker of demyelination PMID: 16374777
TNFalpha-induced NF kappaB pathway contributes to the induction of p8 during pancreatitis and lipopolysaccharide-induced inflammation. PMID: 16981138
Results show that p8-expressing C-LbetaT2 cells form tumors at a rate five-times faster than p8-knockdown cells. PMID: 17451874
Loss of the protein NUPR1 (p8) leads to delayed LHB expression, delayed ovarian maturation, and testicular development of a sertoli-cell-only syndrome-like phenotype in mice. PMID: 18495683
nuclear protein 1 (NUPR1) has a role in cancer progression [review] PMID: 19153668
p8 negatively regulates the myoblast cell cycle. PMID: 19723804

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region.

Protein Families

NUPR family

Tissue Specificity

Highly expressed in pancreas and both ovaries and testes.

Database Links

KEGG: mmu:56312

STRING: 10090.ENSMUSP00000032961

UniGene: Mm.18742

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Recombinant Mouse Nuclear protein 1 (Nupr1)

Product Details

Customer Reviews and Q&A

Target Background

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