Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nme1

Uniprot No.

P15532

Alternative Names

Nme1; Nm23; Nucleoside diphosphate kinase A; NDK A; NDP kinase A; EC 2.7.4.6; Metastasis inhibition factor NM23; NDPK-A; Tumor metastatic process-associated protein; nm23-M1

Species

Mus musculus (Mouse)

Expression Region

2-152

Target Protein Sequence

ANSERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE HYTDLKDRPF FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFQPEELV EYKSCAQNWI YE

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Gene References into Functions

Deletion of NME1 reduced total NDPK activity and exacerbated activation of the stress-related MAPK, JNK, in the liver in response to paraquat. NDPK activity protects cells from acute oxidative stress by inhibiting activation of JNK in mammal models. PMID: 28077425
data suggested that NME1 acts as a switcher or reprogramming factor which involves in oligodentrocyte versus neuron cell fate specification in vitro PMID: 23023023
NM23 deficiency promotes metastasis in a UV radiation-induced mouse model of human melanoma. PMID: 22699362
NME1 controls annexin IV and EF-1Balpha amounts by post-translational mechanisms. PMID: 21541759
data show a critical role for NM23 isoforms in limiting mutagenesis and suppressing UVR-induced melanomagenesis PMID: 22080566
GAPDH and NDPK are genetic modifiers of murine DDC-induced liver injury PMID: 22006949
The work provides a rare instance of nm23-1/NDPKA physiological functions in the mammary glands and reveals its implication as a modulator factor of proliferation and apoptosis in this tissue. PMID: 21490937
Ha-ras oncogene regulates morphogenesis, tumorigenesis, and metastasis through suppressing nm23 expression and modulation of immune cell function PMID: 20944141
Data suggested that nm23-M1/NDPK A was involved in the process of blastocyst implantation. PMID: 15511071
suppressor of tumorigenicity 7 (ST7) and nonmetastatic 23 (NM23) are direct targets of PRMT5-containing BRG1 and hBRM complexes PMID: 15485929
NDPKB-dependent nm23-M1 gene (NDPKA) transactivation requires elements different from those involved in c-myc transactivation. cell death induced by c-myc overexpression or H(2)O(2) exposure was decreased in nm23-transfected compared to control BAF3 cells PMID: 15530864
The lack of NM23-M1 expression promotes metastasis in the SV40 animal model of liver carcinogenesis. PMID: 15928304
The data provide novel insight into the subunit composition of the epithelial CFTR/AMPK/NDPK complex, such that: CFTR interacts specifically with AMPK alpha1, gamma2 and NDPK-A and not NDPK-B or AMPK gamma1. PMID: 16466905
TfR1 is a downstream target of NDPKs and that reduced iron in Nme1(-/-)/Nme2(-/-) erythroblasts is inhibiting their development PMID: 19235734
Data show that embryonic fibroblasts from wild-type and NDPK A/B knockout mice demonstrate a similar reduction of G protein in mammalian cells. PMID: 19805292

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Involvement in disease

This protein is found in reduced amount in tumor cells of high metastatic potential.

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

NDK family

Database Links

KEGG: mmu:18102

STRING: 10090.ENSMUSP00000117022

UniGene: Mm.439702

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Recombinant Mouse Nucleoside diphosphate kinase A (Nme1)

Product Details

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Target Background

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