Recombinant Mouse Phakinin (Bfsp2)

Code CSB-YP754142MO
MSDS
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Source Yeast
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Code CSB-EP754142MO
MSDS
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Source E.coli
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Code CSB-EP754142MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP754142MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP754142MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bfsp2
Uniprot No.
Alternative Names
Bfsp2Phakinin; 49 kDa cytoskeletal protein; Beaded filament structural protein 2; Lens fiber cell beaded filament protein CP 47; CP47; Lens fiber cell beaded filament protein CP 49; CP49; Lens intermediate filament-like light; LIFL-L
Species
Mus musculus (Mouse)
Expression Region
1-416
Target Protein Sequence
MSKRRVAADL PSGTNSSMPV QRHRVSSLRG THSPSSLDSP PASRTSAVGS LVRAPGVYVG VAPSGGIGGL GARVTRRALG ISSVFLQGLR SSGLANVPAP GPERDHTTVE DLGGCLVEYM TKVHALEQVS QELETQLRAH LESKAKSSGG WDALRASWAS SYQQVGEAVL ENARLLLQME TIQAGADDFK ERYENEQPFR KAAEEEVSSL YKVIDEANLT KTDLEHQIES LKEELGFLSR SYEEDVKVLY KQLAGSELEQ ADVPMGTGLD DVLETIRVQW ERDVEKNRAE AGALLQAKQQ TEVVHVSQTQ EEKLAAALSV ELHDTSRQVQ SLQAETESLR ALKRGLENSL HDAQHWHDME LQNLGAVVGR LEAELAEIRS ETEQQQQERA HLLACKSQLQ KDVASYHALL DREENN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA. Plays a role in maintenance of retinal lens optical clarity.
Gene References into Functions
  1. CP49 and filensin have been considered robust markers for mouse lens fiber cell differentiation. The data reported here, however, document both proteins in the mouse lens epithelium, but only after 5 weeks of age, when lens epithelial growth and mitotic activity have slowed. PMID: 27559293
  2. CP49 is involved in a functional synergy critical for regulating lens fiber cell geometry, transparency, and mechanical stiffness. PMID: 23144950
  3. disruption of the Hsf4 gene leads to cataracts via at least three pathways: down-regulation of gamma-crystallin, particularly gamma S-crystallin; decreased lens beaded filament expression; and loss of post-translational modification of alpha A-crystallin PMID: 19224648
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
Protein Families
Intermediate filament family
Tissue Specificity
Detected in retina lens fiber cells (at protein level). Also expressed in the lens epithelium, abundantly expressed in the anterior and anterolateral epithelium, less frequently expressed nearer the lens coronal equator (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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