Recombinant Mouse Probable E3 ubiquitin-protein ligase MID2 (Mid2)

Code CSB-YP865569MO
MSDS
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Source Yeast
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Code CSB-EP865569MO
MSDS
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Source E.coli
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Code CSB-EP865569MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865569MO
MSDS
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Source Baculovirus
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Code CSB-MP865569MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mid2
Uniprot No.
Alternative Names
Mid2; Fxy2; Trim1; Probable E3 ubiquitin-protein ligase MID2; EC 2.3.2.27; Midline defect 2; Midline-2; RING-type E3 ubiquitin transferase MID2; Tripartite motif-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-705
Target Protein Sequence
MGESPASAVL NASAGLFSLK METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS SCSSGESIEP ITAFQCPTCR YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES RRERTYRPSS AMSSERIACQ FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR LVEPVSDTHL RGITCLDHEN EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK QTLEMNLTNL VKRNSELENQ MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI AVKIKETKVM KLRKLAQQVA NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA MATASSQVLI PDINFNDAFE NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT VHWISDDEFS ISSYELQYTI FTGQANFISL YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK TNSQPFKLDP KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC HYWEVVMGSS TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPQ LKRLGVLLDY DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI DYPERQECNC RPQESPYVSG MKACH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in microtubule stabilization.
Gene References into Functions
  1. both MID1 and MID2 play a central role in the regulation of granule exocytosis and that functional redundancy exists between MID1 and MID2 in cytotoxic T cells PMID: 25924778
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Protein Families
TRIM/RBCC family
Tissue Specificity
Low abundance in brain and lung, with even lower levels in heart, liver, and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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