Recombinant Mouse Probable proline dehydrogenase 2 (Prodh2)

Code CSB-YP848679MO
MSDS
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Source Yeast
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Code CSB-EP848679MO
MSDS
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Source E.coli
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Code CSB-EP848679MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848679MO
MSDS
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Source Baculovirus
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Code CSB-MP848679MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prodh2
Uniprot No.
Alternative Names
Prodh2; Hypdh; Hydroxyproline dehydrogenase; HYPDH; EC 1.5.5.3; Kidney and liver proline oxidase 1; MmPOX1; Probable proline dehydrogenase 2; EC 1.5.5.2; Probable proline oxidase 2; Proline oxidase-like protein; PO; Proline oxidase
Species
Mus musculus (Mouse)
Expression Region
1-456
Target Protein Sequence
MIWTRLPLYG PSKPSTGGWQ PLRFDGGAFH VKGTAELARA LLVLRLCAWP PLVTHGLAFQ AWSQRLLGSR LSGALLRASI YGQFVAGETA EEVRNCVGQL QALGLQPLLA VPTEEEPDST AKTSEVWYEE NLSAMLRCVD LSRALVDAHG PARNSLMQLK VTALASTRLC KELSAWIQRP RGSSELSPER LAEAMDSGRN LQLSCLSTEQ NQHLQASLSR LHRVAQHARA KCVRLLVDAE YTFINPALSL LVAALAVRWN SPEEGGPWVW NTYQAYLKDT HQRLEQDAEA AHKAGLAFGV KLVRGAYLDK ERSMTQLQGK EDCTQPDYEA TSRSYSRCLE LMLRCVSNHG PPCHLMVASH NEESVRQATK RMWELGIPLD GPVCFGQLLG MCDHVSLALG QAGYMVYKSI PYGCLEEVIP YLIRRAQENR SVLQGARREQ ALLSQELWRR LLGRTA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. Can also use proline as a substrate but with a very much lower efficiency. Does not react with other diastereomers of Hyp: trans-4-D-hydroxyproline and cis-4-L-hydroxyproline. Ubiquininone analogs such as menadione, duroquinone and ubiquinone-1 react more efficiently than oxygen as the terminal electron acceptor during catalysis.
Gene References into Functions
  1. The nutrient starvation elicits increased activity of mitochondrial proline oxidase/dehydrogenase (POX/PRODH) that is causal in triggering a ROS-dependent induction of ATGL. PMID: 24096872
  2. study revealed that the expression of PO and PDH was regulated by HNF1alpha and HNF4alpha PMID: 15581617
Protein Families
Proline oxidase family
Database Links
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