Recombinant Mouse Protein 4.1 (Epb41), partial

Code CSB-YP007706MO
MSDS
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Source Yeast
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Code CSB-EP007706MO
MSDS
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Source E.coli
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Code CSB-EP007706MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007706MO
MSDS
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Source Baculovirus
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Code CSB-MP007706MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Epb41
Uniprot No.
Alternative Names
Epb41; Epb4.1; Kiaa4056Protein 4.1; P4.1; 4.1R; Band 4.1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Gene References into Functions
  1. 4.1R plays an important role in transporting 5-aminolevulinic acid into cells via at least partly interaction with GAT1 and GAT2 transporters in 5-ALA-PDT. PMID: 28881432
  2. This suggests that 4.1R may influence myogenesis by preventing VHL-mediated myogenin degradation. PMID: 27780863
  3. The proportion of 4.1R-positive cells was significantly higher in the HF group than in the controls, which was confirmed by the positive mRNA expression of 4.1R. 4.1R localized mostly to the plasma membrane of myocardial cells and was upregulated with the progression of HF. This suggests that 4.1R may be associated with HF progression. PMID: 27323040
  4. suggest that 4.1R can prevent pathogenic autoimmunity in MS/EAE progression by suppressing the CD4(+) T cell activation PMID: 25243644
  5. a functional role for 4.1R in small intestinal calcium absorption through regulation of membrane expression of PMCA1b. PMID: 23460639
  6. At the intercalated disc 4.1R does not colocalise with the adherens junction protein, beta-catenin, but does overlap with the other plasma membrane signalling proteins, the Na/K-ATPase and the Na/Ca exchanger NCX1. PMID: 22429617
  7. Cell adhesion and signaling pathways regulated by alphavbeta8 integrin and Band 4.1B are essential for normal formation and function of the heart during embryogenesis. PMID: 21181944
  8. A conserved exonic splicing silencer element (CE(16)) in protein 4.1R exon 16 (E16) interacts with hnRNP A/B proteins & plays a role in repression of E16 splicing during early erythropoiesis PMID: 12426391
  9. We speculate that over the repetitive cycles of heart muscle contraction and relaxation, 4.1R is likely to locate, support, and coordinate functioning of key membrane-bound macromolecular assemblies.[Epb41] PMID: 15834631
  10. These results suggest that erythroid protein 4.1 plays a major role in volume regulation and physiologically downregulates Na/H exchange in mouse erythrocytes. PMID: 16774987
  11. 4.1R organizes a macromolecular complex of skeletal and transmembrane proteins at the junctional node. PMID: 18524950
  12. Cytoskeletal protein 4.1R affects repolarization and regulates calcium handling in the heart. PMID: 18787192
  13. 4.1R knockout mice exhibit bradycardia, prolonged action potentials & QT intervals, and anomalous ion channel activities. 4.1R has a role in the cellular accumulation of NaV1.5. Review. PMID: 19272819
  14. 4.1R gene expression involves transcriptional regulation coupled with a complex splicing regulatory network. PMID: 19729518

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
Database Links
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