Recombinant Mouse Protein THEMIS2 (Themis2)

Code CSB-YP849685MO
MSDS
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Source Yeast
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Code CSB-EP849685MO
MSDS
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Source E.coli
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Code CSB-EP849685MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP849685MO
MSDS
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Source Baculovirus
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Code CSB-MP849685MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Themis2
Uniprot No.
Alternative Names
Themis2; Icb1; Protein THEMIS2; Induced by contact to basement membrane 1 protein; Protein ICB-1; Thymocyte-expressed molecule involved in selection protein 2
Species
Mus musculus (Mouse)
Expression Region
1-663
Target Protein Sequence
MEPVPLQDFV SGLDPTSLPR VLRVCSGVYF EGSVYELFGN ECCLSTGDLI KVTHVQLQKV VCEYPETGQT LELNPNFTGL FSPLTSLRSY RTLEDLVSAM PQNSTRWPIY FKSTQRIVTK ASVVPEDQPL RLEAVEIHHG IRYARCVQVS KTKELLHLPL SQKGPFWRCK PSAPQTLHQI LQDPALKDLT LSCPSLPWNS VILKPQYMLQ AIMHMRSSIV KIPSTLEVEV EDVTASSQHI HFFKPLRLSE VLAGGGPFPL TTEILEVPEG PPVFLSPWVS FLRKGQRLCI YGPASPSWRV VASSKSRKVP RYFMLSGAYQ GKLKRRPREF STAYDLLGAL QPGRPLRVVA TKDCDGNEEE NPDFSFLAVG DRLEVLRSGQ VCGTKGQDID VLVCQRLSEQ SGEEEEDLEE IEDEAEDKEQ ILLPLYLSGS FVEEVNDSRR YNLVDLTAQY SLPCEVKVVT KDTRHPTDPL ASFPGLRLEE KLTEPFLVVS LDSQPEMCFE IPPRWLDLTV VEAEGQPAQV ARPLSIAPVE ELSEAFYYSL RKLPASESQA PPPRPPKSQG INKKQQNIQS CKESSVKPQV VEPQKSCPQP QLKAKTLEAL PKNKSNVYSK ISVHKKDRKP NPQTQNSVLS MKPKTSSSLG KHSTMESHLL PDPDMDDHDY EEI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways.
Gene References into Functions
  1. this stuidy identifies a unique function for Themis2 in differential signaling and provide insight into how B cells discriminate between antigens of different quantity and quality PMID: 27992403
  2. Data indicate that Themis2 protein is not required for B cell development, for activation, or for antibody responses either to model Ags or to influenza viral infection. PMID: 24907343
  3. Study hypothesise that Themis2 may constitute a novel, physiological control point in macrophage inflammatory responses. PMID: 20644716
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Themis family
Tissue Specificity
Expressed in both developing and mature B-cells with high expression in immature, follicular and B1 B cells. Also expressed in macrophages and dendritic cells. Down-regulated in splenocytes of mice developing arthritis in a collagen-induced model, not in
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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