Recombinant Mouse Retrotransposon-like protein 1 (Rtl1), partial

Code CSB-YP762602MO
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Source Yeast
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Code CSB-EP762602MO
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Source E.coli
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Code CSB-EP762602MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP762602MO
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Source Baculovirus
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Code CSB-MP762602MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rtl1
Uniprot No.
Alternative Names
Rtl1; Mar1; Mart1; Peg11Retrotransposon-like protein 1; Mammalian retrotransposon derived protein 1; Paternally expressed gene 11 protein; Retrotransposon-derived protein PEG11
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in capillaries endothelial cells for the maintenance of feto-maternal interface and for development of the placenta.
Gene References into Functions
  1. Paternally expressed 11/Retrotransposon-like 1 (Peg11/Rtl1) knockout (KO) mice show mid- to late fetal lethality or late fetal growth retardation associated with frequent neonatal lethality PMID: 28111885
  2. miR-127 is an essential regulator of Rtl1, mediated by a trans-homologue interaction between reciprocally imprinted genes on the maternally and paternally inherited chromosomes PMID: 26138477
  3. Rtl1, an imprinted gene with preferential expression from the paternal allele, is essential for maintenance of the fetal capillaries. PMID: 18176565
  4. Despite the coordinate down-regulation of Dlk1 and Rtl1 mRNA levels, Rtl1 over-expression did not affect the time course of adipogenesis or Dlk1 expression PMID: 17216273
  5. These data support a role for mir-127 and mir-136 in the epigenetic reprogramming of the Rtl1 imprinting process. PMID: 19126398

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Subcellular Location
Membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed in placenta and in various tissues in late-fetal stage.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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