Recombinant Mouse Secretogranin-1 (Chgb)

Code CSB-YP005345MO
MSDS
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Source Yeast
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Code CSB-EP005345MO
MSDS
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Source E.coli
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Code CSB-EP005345MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005345MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005345MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Chgb
Uniprot No.
Alternative Names
Chgb; Scg-1; Scg1; Secretogranin-1; Chromogranin-B; CgB; Secretogranin I; SgI) [Cleaved into: CCB peptide; PE-11]
Species
Mus musculus (Mouse)
Expression Region
21-677
Target Protein Sequence
APVDNRDHNE EMVTRCIIEV LSNALSKSSV PTITPECRQV LKKSGKEVKG EEKGENQNSK FEVRLLRDPA DASGTRWASS REDAGAPVED SQGQTKVGNE KWTEGGGHSR EGVDDQESLR PSNQQASKEA KIYHSEERVG KEREKEEGKI YPMGEHREDA GEEKKHIEDS GEKPNTFSNK RSEASAKKKD ESVARADAHS MELEEKTHSR EQSSQESGEE TRRQEKPQEL TDQDQSQEES QEGEEGEEGE EGEEGEEDSA SEVTKRRPRH HHGRSGSNKS SYEGHPLSEE RRPSPKESKE ADVATVRLGE KRSHHLAHYR ASEEEPEYGE ESRSYRGLQY RGRGSEEDRA PRPRSEESQE REYKRNHPDS ELESTANRHG EETEEERSYE GANGRQHRGR GREPGAHSAL DTREEKRLLD EGHYPVRESP IDTAKRYPQS KWQEQEKNYL NYGEEGDQGR WWQQEEQLGP EESREEVRFP DRQYEPYPIT EKRKRLGALF NPYFDPLQWK NSDFEKRGNP DDSFLEDEGE DRNGVTLTEK NSFPEYNYDW WERRPFSEDV NWGYEKRSFA RAPQLDLKRQ YDGVAELDQL LHYRKKADEF PDFYDSEEQM GPHQEANDEK ARADQRVLTA EEKKELENLA AMDLELQKIA EKFSQRG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Gene References into Functions
  1. This analysis of mice lacking Cgs highlights the important roles of CgA and CgB in glucose and cardiovascular homeostasis. This study also unveils the existence of direct implications of Cgs in the control of behaviour and mood. PMID: 25257107
  2. Chromogranin B contributes to a highly efficient system that directly mediates monoamine accumulation and exocytosis in large dense core secretory vesicles. PMID: 22415354
  3. Although chromogranins regulate the amine concentration in LDCVs, they are not completely essential. PMID: 21990378
  4. C-terminal domain of chromogranin B regulates intracellular calcium signaling. PMID: 22016391
  5. CgB is not required for normal insulin granule biogenesis or maintenance in vivo, but is essential for adequate secretion of islet hormones PMID: 20126668
  6. Chromogranin B gene ablation reduces the catecholamine cargo and decelerates exocytosis in chromaffin secretory vesicles in mice. PMID: 20089903
  7. CGB was localized in the nucleus; transcription control role of CGB in the nucleus PMID: 11854265
  8. Chromogranin B has a role in secretory granule biogenesis PMID: 12902350
  9. CGB may play an important modulatory role in the control of Ca2+ release from the endoplasmic reticulum PMID: 14506248
  10. Neuroendocrine-specific expression of Chgb is mediated by the CRE and G/C boxes in cis and the transcription factors CREB, AP-2, Egr-1 and Sp1. PMID: 16987240

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Subcellular Location
Secreted. Note=Neuroendocrine and endocrine secretory granules.
Protein Families
Chromogranin/secretogranin protein family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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