Recombinant Mouse Synembryn-B (Ric8b)

Code CSB-YP773932MO
MSDS
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Source Yeast
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Code CSB-EP773932MO
MSDS
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Source E.coli
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Code CSB-EP773932MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773932MO
MSDS
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Source Baculovirus
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Code CSB-MP773932MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ric8b
Uniprot No.
Alternative Names
Ric8b; Synembryn-B; Protein Ric-8B
Species
Mus musculus (Mouse)
Expression Region
1-520
Target Protein Sequence
MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESADE DKRKKLCEGI FKVLVKEVPT TCQVSCLEVL RILSRDKKIL VPVTTKENMQ ILLRLAKLHE SDDSLEKVSE FPVIVESLKC LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFV LSLLHTDIRS QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS PQETDCAIEA LKALFNVTVD SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL THEETAQEAA TLDELPSDKT TEKDTALKNS TMVYNGMNME AIHVLLNFME KRIDKGSSYR EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN AKPKEELLKP MGLKPDGTIT PLEEALSQYS VIEETSSDTD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. Able to potentiate G(olf)-alpha-dependent cAMP accumulation suggesting that it may be an important component for odorant signal transduction.
Gene References into Functions
  1. Olfactory-specific Ric-8b knock-out mice of mixed sex do not express the Galphaolf protein in the olfactory epithelium. The mature olfactory sensory neuron layer is reduced and cell death increased. These mice have an impaired sense of smell. Ric-8b is essential for olfactory function, and it may also be essential for Galphaolf-dependent functions in the brain. PMID: 29118104
  2. Results indicate that a functional N-terminus in C/EBPbeta-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts. PMID: 24585571
  3. a new role of Ric-8B in the crosstalk of two distinct G protein signaling pathways, which are possibly involved in a part of mechanisms of chronic heart failure. PMID: 24134321
  4. Data show that the Ric-8B gene is negatively regulated during osteoblast differentiation by the transcription factor C/EBPbeta. PMID: 21606199
  5. Ric-8BFL-catalyzed nucleotide exchange probably proceeds in the forward direction to produce Galpha-GTP in cells. PMID: 21467038
  6. Ric-8B plays a critical and specific role in the control of G alpha(s) protein levels by modulating G alpha(s) ubiquitination and positively regulates G(s) signaling PMID: 20133939
  7. Results show that Ric-8B, a putative guanine nucleotide exchange factor for Galphaolf, promotes efficient heterologous expression of odorant receptors. PMID: 16754875
  8. Ric-8B interacts with two different olfactory G protein subunits and is implicated as an important protein in odorant signal transduction. PMID: 18462949

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Subcellular Location
Cytoplasm, cell cortex.
Protein Families
Synembryn family
Tissue Specificity
Predominantly expressed in the mature olfactory sensory neurons and also in a few regions in the brain.
Database Links

KEGG: mmu:237422

UniGene: Mm.18936

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