Recombinant Mycobacterium smegmatis 50S ribosomal protein L6(rplF)

Code CSB-YP367970MVX
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Source Yeast
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Code CSB-EP367970MVX
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Source E.coli
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Code CSB-EP367970MVX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP367970MVX
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Source Baculovirus
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Code CSB-MP367970MVX
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names rplF
Uniprot No. A0QSG4
Alternative Names rplF; MSMEG_1470; MSMEI_1434; 50S ribosomal protein L6
Species Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Expression Region 1-179
Target Protein Sequence MSRIGKQPVP VPSGVDVTIN GQNLSVKGPK GTLTLDVAEP ISVSRAEDGA IVVTRPDDER RSRSLHGLSR TLIANLVTGV TEGYTQKMEI FGVGYRVQLK GQNLEFALGY SHPVLIEAPE GITFAVESPT KFSVSGIDKQ KVGQISAVIR RLRRPDPYKG KGVRYEGEQI RRKVGKTGK
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.
Protein Families Universal ribosomal protein uL6 family
Database Links

KEGG: msb:LJ00_07345

STRING: 246196.MSMEG_1470

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