Recombinant Mycobacterium smegmatis Nucleoside triphosphate pyrophosphohydrolase (mazG)

Code CSB-YP369873MVX
MSDS
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Source Yeast
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Code CSB-EP369873MVX
MSDS
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Source E.coli
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Code CSB-EP369873MVX-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP369873MVX
MSDS
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Source Baculovirus
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Code CSB-MP369873MVX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mazG
Uniprot No.
Alternative Names
mazG; MSMEG_5422; MSMEI_5273Nucleoside triphosphate pyrophosphohydrolase; NTP-PPase; EC 3.6.1.8
Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Expression Region
1-324
Target Protein Sequence
MIVVLVDPRR PALVPVDAVE FLTGDVQYTE EMPVKVPWSL PSARPAYDGE DAPVLLSSDP EHPVVKARLA AGDRLIAAPE PQPGERLVDA VALMDKLRTS GPWESEQTHD SLRRYLLEET YELFDAVRSG NADELREELG DVLLQVLFHA RIAEDAPHHP FSIDDVADAL VRKLGNRVPA VLAGESISLD EQLAQWEERK AQEKKVKARA SSMDDVPTGQ PALALAQKVL ARVSQAGLPA ELIPASLTSV SVSADTDSEN ELRTAVLEFM DTVREVEAAV AAGRRGEDVP EELDVAPLGV ISEDEWRAYW PGAESSASEA EPEE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required to maintain the full capacity of the mycobacteria to respond to oxidative stress via the degradation of the oxidation-induced damaged nucleotides. It hydrolyzes all canonical (d)NTPs, as well as the mutagenic dUTP and 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP). Also involved in the transcriptional activation of RelA in response to oxidative stress.
Protein Families
Nucleoside triphosphate pyrophosphohydrolase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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