Recombinant Naja atra L-amino-acid oxidase

Code CSB-YP011660NFG
MSDS
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Source Yeast
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Code CSB-EP011660NFG
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Source E.coli
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Code CSB-EP011660NFG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011660NFG
MSDS
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Source Baculovirus
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Code CSB-MP011660NFG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
L-amino-acid oxidase; LAO; NA-LAAO; EC 1.4.3.2; Fragment
Species
Naja atra (Chinese cobra)
Expression Region
20-449
Target Protein Sequence
D DRRSPLEECF QQNDYEEILE IARNGLKKTS NPKHVVVVGA GMAGLSAAYV LAGAGHKVTL LEASERVGGR VITYHNDREG WYVNMGPMRL PERHRIVREY IRKFGLKLNE FFQENENAWY YINNIRKRVW EVKKDPSLLK YPVKPSEEGK SASQLYQEPL RKVIEELKRT NCSYILNKYD SYSTKEYLIK EGNLSRGAVD MIGDLLNEDS SYHLSFMESL KSDALFSYEK RFDEIVGGFD QLPISMYQAI AEMVHLNARV IKIQYDAEKV RVTYQTPAKT FVTADYVIVC STSRAARRIY FEPPLPPKKA HALRSIHYRS ATKIFLTCSK KFWEADGIHG GKSTTDLPSR FIHYPNHNFT SGIGVIMAYV LADDSDFFQA LDTKTCADIV INDLSLIHDL PKREIQALCY PSIKKWNLDK YTMGSITSF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Shows activity on L-Leu. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities. This protein induces platelet aggregation by both hydrogen peroxide production and binding to platelet membrane proteins (that would enhance the sensitivity of platelets to hydrogen peroxide). Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.
Subcellular Location
Secreted.
Protein Families
Flavin monoamine oxidase family, FIG1 subfamily
Tissue Specificity
Expressed by the venom gland.
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