Recombinant Neurospora crassa Probable tRNA-splicing endonuclease subunit sen-34 (sen-34)

Code CSB-YP755943NHA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP755943NHA
MSDS
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Source E.coli
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Code CSB-EP755943NHA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP755943NHA
MSDS
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Source Baculovirus
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Code CSB-MP755943NHA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tsp-4
Uniprot No.
Alternative Names
tsp-4; sen34; B2E7.010; NCU08032; Probable tRNA-splicing endonuclease subunit tsp-4; EC 4.6.1.16; tRNA-intron endonuclease sen34; tRNA-splicing protein 2
Species
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Expression Region
1-315
Target Protein Sequence
MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ NVFLGLPLEL YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ SIKTQRRTAQ LVFNEAKAQS MAKHKDKRKP KQPQPSVVVP TELPLSASSA DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD ASTPPQKPLV KEKLPAITPT TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF GGDFSVYPGD PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS EAGELRAFCI EWAGM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.
Protein Families
TRNA-intron endonuclease family
Database Links

KEGG: ncr:NCU08032

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