Recombinant Phaeosphaeria nodorum Cytoplasmic tRNA 2-thiolation protein 2(NCS2)

Product Type Recombinant Protein
Uniprot No. Q0UDH1
Abbreviation NCS2
Species Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Parastagonospora nodorum)
Gene Names NCS2
Protein Names Recommended name: Cytoplasmic tRNA 2-thiolation protein 2
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Subcellular Location Cytoplasm
Protein Families CTU2/NCS2 family
Database Links

KEGG: pno:SNOG_10193

STRING: 13684.SNOT_10193

Product Details for Different Expression Systems

Product Name Recombinant Phaeosphaeria nodorum Cytoplasmic tRNA 2-thiolation protein 2(NCS2),Yeast
Code CSB-YP604332EUG
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPGKHSDSAT SELCEKCKEN ISILVVRAKP ICHDCFARYV HTKAIKRLES FRVNFAASPD QQRKILLPLS HGVSSTTLLH ILDLHLNTQR SKTNRTGFAI SVLVVDEGSL DPARLDKVRE RYANHDYASL PLHDVFRLLP DDASLRAFLP ESQVQEVCST QEQLTSLIAS LTSATARADV LTTLRTRLIV EHAKQTGCES ILWGDSTTRL AEKTLAETAK GRGFSLPWQI SDGKSPFDIN FHYPLRDVLK KELFSYVDLA EPALSALVYE PQSGATQASM SSKNTTIDDL MKQYFESVEE NFPSIVSNVV RTTGKLEVPT GATSNPRCSL CGMPVPDGRF GIHGWGGDQH GGADDAAAVS GGSLCYGCTR SIPQRGTAVA SK
Expression Region 1-382
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Phaeosphaeria nodorum Cytoplasmic tRNA 2-thiolation protein 2(NCS2),E.coli
Code CSB-EP604332EUG
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPGKHSDSAT SELCEKCKEN ISILVVRAKP ICHDCFARYV HTKAIKRLES FRVNFAASPD QQRKILLPLS HGVSSTTLLH ILDLHLNTQR SKTNRTGFAI SVLVVDEGSL DPARLDKVRE RYANHDYASL PLHDVFRLLP DDASLRAFLP ESQVQEVCST QEQLTSLIAS LTSATARADV LTTLRTRLIV EHAKQTGCES ILWGDSTTRL AEKTLAETAK GRGFSLPWQI SDGKSPFDIN FHYPLRDVLK KELFSYVDLA EPALSALVYE PQSGATQASM SSKNTTIDDL MKQYFESVEE NFPSIVSNVV RTTGKLEVPT GATSNPRCSL CGMPVPDGRF GIHGWGGDQH GGADDAAAVS GGSLCYGCTR SIPQRGTAVA SK
Expression Region 1-382
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Phaeosphaeria nodorum Cytoplasmic tRNA 2-thiolation protein 2(NCS2),Baculovirus
Code CSB-BP604332EUG
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPGKHSDSAT SELCEKCKEN ISILVVRAKP ICHDCFARYV HTKAIKRLES FRVNFAASPD QQRKILLPLS HGVSSTTLLH ILDLHLNTQR SKTNRTGFAI SVLVVDEGSL DPARLDKVRE RYANHDYASL PLHDVFRLLP DDASLRAFLP ESQVQEVCST QEQLTSLIAS LTSATARADV LTTLRTRLIV EHAKQTGCES ILWGDSTTRL AEKTLAETAK GRGFSLPWQI SDGKSPFDIN FHYPLRDVLK KELFSYVDLA EPALSALVYE PQSGATQASM SSKNTTIDDL MKQYFESVEE NFPSIVSNVV RTTGKLEVPT GATSNPRCSL CGMPVPDGRF GIHGWGGDQH GGADDAAAVS GGSLCYGCTR SIPQRGTAVA SK
Expression Region 1-382
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Phaeosphaeria nodorum Cytoplasmic tRNA 2-thiolation protein 2(NCS2),Mammalian cell
Code CSB-MP604332EUG
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPGKHSDSAT SELCEKCKEN ISILVVRAKP ICHDCFARYV HTKAIKRLES FRVNFAASPD QQRKILLPLS HGVSSTTLLH ILDLHLNTQR SKTNRTGFAI SVLVVDEGSL DPARLDKVRE RYANHDYASL PLHDVFRLLP DDASLRAFLP ESQVQEVCST QEQLTSLIAS LTSATARADV LTTLRTRLIV EHAKQTGCES ILWGDSTTRL AEKTLAETAK GRGFSLPWQI SDGKSPFDIN FHYPLRDVLK KELFSYVDLA EPALSALVYE PQSGATQASM SSKNTTIDDL MKQYFESVEE NFPSIVSNVV RTTGKLEVPT GATSNPRCSL CGMPVPDGRF GIHGWGGDQH GGADDAAAVS GGSLCYGCTR SIPQRGTAVA SK
Expression Region 1-382
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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