Recombinant Pig Major seminal plasma glycoprotein PSP-I

Code CSB-YP339558PI
MSDS
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Source Yeast
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Code CSB-EP339558PI
MSDS
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Source E.coli
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Code CSB-EP339558PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339558PI
MSDS
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Source Baculovirus
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Code CSB-MP339558PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Major seminal plasma glycoprotein PSP-I; SP3
Species
Sus scrofa (Pig)
Expression Region
25-133
Target Protein Sequence
LDYHAC GGRLTDDYGT IFTYKGPKTE CVWTLQVDPK YKLLVSIPTL NLTCGKEYVE ILEGAPGSKS LGKFCEGLSI LNRGSSGMTV KYKRDSGHPA SPYEIIFLRD SQG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Not yet identified, major porcine seminal plasma protein. Can bind soybean trypsin inhibitor after deglycosylation.
Gene References into Functions
  1. PSP-I/PSP-II heterodimer in seminal plasma has a predominant role in triggering the recruitment of uterine polymorphonuclear neutrophils and T cells after mating, initiating a cascade of transient and long-lasting immunological events. PMID: 19948361
  2. No obvious influence of heterodimer on capability of freshly extended boar spermatozoa to penetrate homologous oocytes. PSP-I/PSP-II exerted deleterious effect when frozen-thawed spermatozoa were used to penetrate in vitro matured oocytes. PMID: 15477376
  3. the protective effect of the PSP-I/PSP-II heterodimer in spermatozoa appears to be related to its adhesion to the acrosomal area, and the loss of this protective effect coincides with a stepwise redistribution of PSP-I/PSP-II during incubation PMID: 16809275
  4. The modulation of the structural organization and heparin-binding ability of PSP-I by Zn2+ might be a physiological phenomenon in seminal plasma. PMID: 16819821
  5. results indicate that the seminal vesicles are the main source of PSP-I and PSP-II spermadhesins, although epididymal segments, testis and the bulbourethral gland also participate in the expression of both proteins PMID: 17651403

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Subcellular Location
Secreted.
Protein Families
Spermadhesin family
Tissue Specificity
Seminal plasma or sperm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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