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Recombinant Pseudomonas aeruginosa Cyclic AMP receptor-like protein (vfr)

Recombinant Pseudomonas aeruginosa Cyclic AMP receptor-like protein (vfr)

Code	CSB-YP346227EZX
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP346227EZX
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP346227EZX-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP346227EZX
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP346227EZX
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

vfr

Uniprot No.

P55222

Alternative Names

vfr; PA0652; Cyclic AMP receptor-like protein

Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Expression Region

1-214

Target Protein Sequence

MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL VHVKGKTMVV FGTR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Global cAMP-dependent transcriptional regulator that controls virulence gene expression by distinct cAMP-dependent and -independent mechanisms, which allow to fine tune its virulence program in response to specific host cues or environments. Controls the expression of many regulatory targets including type II, type III and type IV secretion systems, flagellar-mediated motility, and quorum sensing systems. Transcriptional control is exerted by binding to a well-characterized consensus site (5'-ANWWTGNGAWNYAGWTCACAT) within target promoters. Directly binds to the toxA upstream region to regulate exotoxin A production, to the lasR gene promoter to activate the las quorum-sensing system or to the exsA promoter to regulate type III secretion system. Autoregulates as well its own expression.

Gene References into Functions

Vfr directly regulates rhlR transcription through its binding to several Vfr-binding sites (VBSs) present in the rhlR promoter region. PMID: 21719541
The crystal structure of Vfr shows that it is a winged-helix DNA-binding protein like its homologue cyclic AMP receptor protein (CRP). PMID: 21665969
study suggests that Vfr shares many of the same functions as cAMP receptor protein, CRP, but has specialized functions, at least in terms of DNA target sequence binding, required for regulation of a subset of genes in its regulon PMID: 17159200
In a reconstruction experiment conducted with a 1:1 mixture of wild-type and a vfr mutant of PAO1, data showed that the vfr mutant had a selective advantage over the wild type after growth in static culture for 4 days. PMID: 18203852
The results suggest that Vfr modulates toxA and lasB expression in PAO1 through PtxR; a model defining the relationships between these different genes is presented. PMID: 18227247
Vfr enhances toxA expression in PAO1 both directly and indirectly through regA, but not through pvdS;vfr expression is not regulated by iron;both Vfr and PvdS cooperate in the presence of RegA to achieve a maximum level of toxA expression. PMID: 19389782