Recombinant Pseudomonas aeruginosa RNA polymerase sigma-54 factor (rpoN)

Code CSB-YP343451EZX
MSDS
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Source Yeast
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Code CSB-EP343451EZX
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Source E.coli
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Code CSB-EP343451EZX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP343451EZX
MSDS
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Source Baculovirus
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Code CSB-MP343451EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rpoN
Uniprot No.
Alternative Names
rpoN; PA4462; RNA polymerase sigma-54 factor
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-497
Target Protein Sequence
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGDDFDNSD PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS ELPVDTAWED IYQTSASSLP SNDDDEWDFT ARTSSGESLH SHLLWQVNLA PMSDTDRMIA VTIIDSINND GYLEESLEEI LAAIDPELDV ELDEVEVVLR RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV SDYLDLLGGR DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI KSLQSRNETL MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIFELK YFFSSHVSTA EGGECSSTAI RAIIKKLVAA ENAKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Plays a role in the regulation of many virulence factors, motility, quorum sensing, mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria, and biofilm formation. Positively controls the T6 secretion system by directly binding to the promoter regions of hcpA and hcpB genes, leading to their expression. Thereby, allows to colonize several hosts efficiently including mammals, insects, nematodes and plants.
Gene References into Functions
  1. RpoN interacts with pqsA, pqsE, pqsH, and rhlR in response to antibiotic stress.RpoN negatively regulates the expression of Pseudomonas quinolone signal. PMID: 27431228
  2. Together, these data strongly suggest that KinB regulates virulence factors important for the development of acute pneumonia and conversion to mucoidy via RpoN. PMID: 22210761
  3. RpoN negatively regulates aceA activity. PMID: 20093293
Protein Families
Sigma-54 factor family
Database Links

KEGG: pae:PA4462

STRING: 208964.PA4462

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