Recombinant Pseudomonas aeruginosa Transcriptional activator protein lasR (lasR)

Code CSB-YP335091EZX
MSDS
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Source Yeast
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Code CSB-EP335091EZX
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Source E.coli
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Code CSB-EP335091EZX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335091EZX
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Source Baculovirus
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Code CSB-MP335091EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lasR
Uniprot No.
Alternative Names
lasR; PA1430; Transcriptional activator protein LasR
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-239
Target Protein Sequence
MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA FIVGNYPAAW REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE FFEEASAAGL VYGLTMPLHG ARGELGALSL SVEAENRAEA NRFMESVLPT LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR EKEVLQWCAI GKTSWEISVI CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.
Gene References into Functions
  1. The results suggest that the strength of transcription could be one of the deterministic factors that drive the mutagenesis of lasR in P. aeruginosa. PMID: 27650847
  2. it is observed that LasR protein binds to the promoter DNA in a better way when it itself is attached to the autoinducer ligand PMID: 26768577
  3. The new data shows that the active form of the LasR dimer binds two signal molecules cooperatively and that the timescale for reaching saturation is independent of the signal molecule concentration. PMID: 23807499
  4. We show an additional regulation of bphP expression through the quorum-sensing (QS) regulator LasR. PMID: 21415115
  5. LasR can fold into an active conformation in vivo in the absence of the acyl-HSL ligand. PMID: 21325039
  6. purified native LasR and characterized binding to various quorum-controlled promoters in vitro PMID: 15505212
  7. MvfR contributes to P. aeruginosa virulence by positively regulating a subset of genes dependant on both LasR and RhlR. PMID: 15686549
  8. effects and interplay of iron concentration and dissolved oxygen tension on the expression of lasR PMID: 15817780
  9. alkaline stress may be a critical factor providing a selective survival advantage to lasR mutants PMID: 15995202
  10. Our results demonstrate that the las system directly regulates vqsR, and certain nucleotides in the las box are crucial for LasR binding and activation of the vqsR promoter. PMID: 17449616
  11. Loss of LasR function may represent a marker of an early stage in chronic infection of the cystic fibrosis airway with clinical implications for antibiotic resistance and disease progression. PMID: 17493132
  12. Transposon mutagenesis of pAHL-BAC confirmed that transcriptional regulation by LasR is necessary for 3OC12HSL production PMID: 17559399
  13. In the absence of LasR the expression of RhlR-regulated factors is only delayed. PMID: 19246742
  14. The findings support the notion that the core function of LasR is to co-ordinate the production of extracellular factors. PMID: 19682264

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Protein Families
Autoinducer-regulated transcriptional regulatory protein family
Database Links

KEGG: pae:PA1430

STRING: 208964.PA1430

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