Recombinant Pseudomonas aeruginosa tRNA (uracil(54)-C(5))-methyltransferase

Product Type Recombinant Protein
Uniprot No. B7V1D2
Species Pseudomonas aeruginosa (strain LESB58)
Gene Names trmA
Protein Names Recommended name: tRNA (uracil(54)-C(5))-methyltransferase EC= 2.1.1.35 Alternative name(s): tRNA(m5U54)-methyltransferase Short name= RUMT
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA).
Protein Families Class I-like SAM-binding methyltransferase superfamily, RNA M5U methyltransferase family, TrmA subfamily
Database Links

KEGG: pag:PLES_51051

Product Details for Different Expression Systems

Product Name Recombinant Pseudomonas aeruginosa tRNA (uracil(54)-C(5))-methyltransferase,Yeast
Code CSB-YP490157EZY
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSRPQFDPSA YAAQLEDKKS RLAGLLAPFA APAPEVFESP REHYRLRAEF RLWRETGNEK RHYAMFEQGD KHTPILIEDF PIASRRINEL MPRLKAAWAD PALGFKLFQV EFLTTLAGDA LITLCYHRPI DDAWRQAAEK LAAELGVNLV GRSRGKRIVV GRDYVEEELS VAGRRFRYRQ PEGAFTQPNG EVNQKMLGWA YEALGQRDDD LLELYCGNGN FTLPLATRVR KVLATEISKS SVNAALANLA DNAVDNVSLV RLSAEELTQA LNEVRPFRRL ADIDLKSYAF GSVFVDPPRA GMDPDTCELA RRFERILYIS CNPETLAQNI AQLHDTHRIS RCALFDQFPY THHMESGVLL ERR
Expression Region 1-363
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Pseudomonas aeruginosa tRNA (uracil(54)-C(5))-methyltransferase,E.coli
Code CSB-EP490157EZY
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSRPQFDPSA YAAQLEDKKS RLAGLLAPFA APAPEVFESP REHYRLRAEF RLWRETGNEK RHYAMFEQGD KHTPILIEDF PIASRRINEL MPRLKAAWAD PALGFKLFQV EFLTTLAGDA LITLCYHRPI DDAWRQAAEK LAAELGVNLV GRSRGKRIVV GRDYVEEELS VAGRRFRYRQ PEGAFTQPNG EVNQKMLGWA YEALGQRDDD LLELYCGNGN FTLPLATRVR KVLATEISKS SVNAALANLA DNAVDNVSLV RLSAEELTQA LNEVRPFRRL ADIDLKSYAF GSVFVDPPRA GMDPDTCELA RRFERILYIS CNPETLAQNI AQLHDTHRIS RCALFDQFPY THHMESGVLL ERR
Expression Region 1-363
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Pseudomonas aeruginosa tRNA (uracil(54)-C(5))-methyltransferase,Baculovirus
Code CSB-BP490157EZY
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSRPQFDPSA YAAQLEDKKS RLAGLLAPFA APAPEVFESP REHYRLRAEF RLWRETGNEK RHYAMFEQGD KHTPILIEDF PIASRRINEL MPRLKAAWAD PALGFKLFQV EFLTTLAGDA LITLCYHRPI DDAWRQAAEK LAAELGVNLV GRSRGKRIVV GRDYVEEELS VAGRRFRYRQ PEGAFTQPNG EVNQKMLGWA YEALGQRDDD LLELYCGNGN FTLPLATRVR KVLATEISKS SVNAALANLA DNAVDNVSLV RLSAEELTQA LNEVRPFRRL ADIDLKSYAF GSVFVDPPRA GMDPDTCELA RRFERILYIS CNPETLAQNI AQLHDTHRIS RCALFDQFPY THHMESGVLL ERR
Expression Region 1-363
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Pseudomonas aeruginosa tRNA (uracil(54)-C(5))-methyltransferase,Mammalian cell
Code CSB-MP490157EZY
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSRPQFDPSA YAAQLEDKKS RLAGLLAPFA APAPEVFESP REHYRLRAEF RLWRETGNEK RHYAMFEQGD KHTPILIEDF PIASRRINEL MPRLKAAWAD PALGFKLFQV EFLTTLAGDA LITLCYHRPI DDAWRQAAEK LAAELGVNLV GRSRGKRIVV GRDYVEEELS VAGRRFRYRQ PEGAFTQPNG EVNQKMLGWA YEALGQRDDD LLELYCGNGN FTLPLATRVR KVLATEISKS SVNAALANLA DNAVDNVSLV RLSAEELTQA LNEVRPFRRL ADIDLKSYAF GSVFVDPPRA GMDPDTCELA RRFERILYIS CNPETLAQNI AQLHDTHRIS RCALFDQFPY THHMESGVLL ERR
Expression Region 1-363
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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