Recombinant Rat Endothelin-3 (Edn3)

Code CSB-YP007402RA
MSDS
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Source Yeast
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Code CSB-EP007402RA
MSDS
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Source E.coli
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Code CSB-EP007402RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007402RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007402RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Edn3
Uniprot No.
Alternative Names
Edn3Endothelin-3; ET-3; Preproendothelin-3; PPET3
Species
Rattus norvegicus (Rat)
Expression Region
53-73
Target Protein Sequence
CTCFTYKD KECVYYCHLD IIW
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. A novel bidirectional interaction exists between endothelin-3 and retinoic acid in rat enteric nervous system precursors. PMID: 24040226
  2. ET-3-induced hemodynamic alterations are mediated by both ET-A and ET-B receptors PMID: 15093706
  3. The pronounced increase of endothelin-3 in the renal medulla is associated with preservation of local Nitric Oxide metabolite levels PMID: 15629454
  4. ET-3 effects on bile secretion are dependent on brain nitric oxide and independent of the autonomic nervous system or hemodynamic variations. PMID: 15949640
  5. Endothelin-3 diminished tyrosine hydroxylase activity in anterior hypothalamus and this effect was blocked by a selective ETB receptor antagonist (100 nM BQ-788), but not by a ET(A) receptor antagonist (BQ-610). PMID: 16023617
  6. Plays an important role in the growth control of colonic epithelial cells, possibly by mediating epithelial-mesenchymal interactions. PMID: 16287488
  7. Present results permit us to conclude that both endothelins play an important role in the regulation of norepinephrine neurotransmission at the presynaptic nerve endings in the hypothalamus. PMID: 18682267
  8. ET-3 significantly contributes to pulmonary vasoconstriction by activating the ET(B) at low concentration, and the ET(A) at high concentration. PMID: 18771698
  9. Present findings show a clear interaction between the endothelin system, and the catecholaminergic transmission in the olfactory bulb. PMID: 18850267

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Subcellular Location
Secreted.
Protein Families
Endothelin/sarafotoxin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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