Recombinant Rat FMRFamide-related peptides (Npff)

Code CSB-YP895764RA
MSDS
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Source Yeast
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Code CSB-EP895764RA
MSDS
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Source E.coli
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Code CSB-EP895764RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895764RA
MSDS
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Source Baculovirus
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Code CSB-MP895764RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Npff
Uniprot No.
Alternative Names
Npff; Pro-FMRFamide-related neuropeptide FF; FMRFamide-related peptides) [Cleaved into: Neuropeptide SF; NPSF); Neuropeptide FF; NPFF); Neuropeptide AF-like; NPAF)]
Species
Rattus norvegicus (Rat)
Expression Region
72-82
Target Protein Sequence
NPAFLFQPQ RF
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF and SF potentiate and sensitize ASIC2 and ASIC3 channels.
Gene References into Functions
  1. we demonstrate, for the first time, that NPFFR2 positively modulates the HPA axis via hypothalamic corticotropin-releasing factor (CRF). Our finding that NPFFR2 regulates stress-dependent neural pathways advances our understanding of neuroendocrine regulation and opens new avenues for probing the functions of NPFF. PMID: 28825666
  2. NPSF may play a physiologic role in the regulation of such circadian functions as the activity of motor centers and the hypothalamic-pituitary-adrenal axis, through the release of corticotropin releasing hormone. PMID: 24316399
  3. the ASIC are tonically active in the vestibular-afferent neurons and modulated by RFamide-like peptides PMID: 22342307
  4. NPFF is capable of up-regulating its own gene expression in the solitary nucleus. NPFF may play a homeostatic role in response to specific cardiovascular challenges, such as hypotension. PMID: 11984823
  5. NPFF mRNA was found in the embryonic spinal cord, caudal solitary tract nucleus, and surprisingly, in the medullary reticular formation. PMID: 12619141
  6. there are both pro- and anti-opioid actions of NPFF in the parabrachial nucleus to modulate feeding. PMID: 14557236
  7. neuropeptide FF is not regulated through the vasopressin regulatory system but via an independent pathway. PMID: 14960341
  8. NPFF facilitates inhibitory input to magnocellular neurosecretory cells of the paraventricular nucleus via GABAergic interneurons located in immediate vicinity of the nucleus. PMID: 16517015
  9. Npff reduces the expression of cocaine-induced conditioned place preference and cocaine-induced sensitization in rats. PMID: 18295932

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Subcellular Location
Secreted.
Protein Families
FARP (FMRFamide related peptide) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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